Deregulated expression of long non-coding RNA UCA1 in multiple myeloma
Deregulated expression of long non-coding RNA UCA1 in multiple myeloma
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DOI：
10.1111/ejh.12908
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发表时间：
2017-09-01
影响因子：
3.1
通讯作者：
Sevcikova, Sabina
中科院分区：
文献类型：
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作者：
Sedlarikova, Lenka;Gromesova, Barbora;Sevcikova, Sabina
目的长非编码RNA(Long Non-Coding RNAs，LncRNAs)是一种长于200个核苷酸的不能翻译成蛋白质的RNA转录本。它们参与了包括癌症在内的许多疾病的发病机制，并有可能作为诊断和预后的标志物。采用三期生物标记物研究新诊断的多发性骨髓瘤(MM)患者和健康献血者(HD)骨髓浆细胞(BMPC)中lncRNA的表达谱。方法用RT-2 lncRNA聚合酶链式反应阵列(QIAGEN)检测83例新诊断MM患者和25例HD患者骨髓浆细胞中LncRNA的表达谱，然后用TaqMan非编码RNA定量实时定量PCR检测84例MM和25例HD患者的LncRNA表达。UCA1水平与血清白蛋白和单抗免疫球蛋白水平、细胞遗传学异常和MM患者的生存期相关。
ObjectivesLong non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study.MethodsExpression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD.ResultsOur analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients.ConclusionOur study suggests a possible prognostic impact of UCA1 expression levels on MM patients.