Identification and characterization of a quantitative trait locus, Hd9, controlling heading date in rice
Identification and characterization of a quantitative trait locus, Hd9, controlling heading date in rice
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DOI：
10.1270/jsbbs.52.35
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发表时间：
2002-03-01
期刊：
影响因子：
2.4
通讯作者：
Yano, M
中科院分区：
文献类型：
--
作者：
Lin, HX;Ashikari, M;Yano, M
以粳稻品种日本晴（Nipponbare）为轮回亲本，籼稻品种Kasalath为供体亲本，在回交BC 4F 2群体中检测到一个新的控制水稻抽穗期的QTL Hd 9。Hd 9被精确定位在3号染色体的短臂上，作为一个单一的孟德尔因子;来自BC 4F 2植株的BC 4F 3系的后代测试显示，它与RFLP标记S12021共分离。此外，利用分子标记辅助选择技术，将Kasalath的一小段染色体片段（包括Hd 9）替换到日本晴的遗传背景中，获得了目标QTL的近等基因系（NIL（110））。NIL（Hd 9）的抽穗天数在长日照和自然田间条件下增加，但在短日照条件下几乎保持相同的同基因对照，日本晴。对NIL（H（19））与NIL（Hd 1）或NIL（Hd 2）杂交的F-2群体的分析表明，H（19）的表型基因效应与Hd 1和Hd 2的表型基因效应是加性的。这一结果表明，没有上位相互作用。在此基础上，我们对Hd 9的生物学功能进行了初步探讨。
A new quantitative trait locus (QTL), Hd9, controlling rice heading date was detected in a BC4F2 backcross population derived from a cross between a japonica rice variety, Nipponbare, as the recurrent parent, and an indica variety, Kasalath, as the donor parent. Hd9 was precisely mapped on the short arm of chromosome 3 as a single Mendelian factor; progeny testing of BC4F3 lines derived from BC4F2 plants revealed that it co-segregated with the RFLP marker S12021. In addition, a nearly isogenic line (NIL) of the target QTL, NIL(110), in which a small chromosomal segment of Kasalath including Hd9 was substituted into the genetic background of Nipponbare, was selected from the progeny based on marker-assisted selection. Days to heading of NIL(Hd9) increased under long-day and natural field conditions at Tsukuba, but remained almost the same under short-day conditions as that of the isogenic control, Nipponbare. Analysis of F-2 populations derived from crosses between NIL(H(19) and either NIL(Hd1) or NIL(Hd2) revealed that the phenotypic gene effect of H(19 was additive to that of lid] and Hd2. This result suggests that no epistatic interaction was involved. Based on these results, we discussed the biological function of Hd9.