Evaluation of an antibody to human milk fat globule antigen in the detection of metastatic carcinoma in pleural, pericardial and peritoneal fluids.
Evaluation of an antibody to human milk fat globule antigen in the detection of metastatic carcinoma in pleural, pericardial and peritoneal fluids.
复制标题
人乳脂肪球抗原抗体在胸膜、心包和腹膜液中转移性癌检测中的评价。
作者：
L. Hilborne;L. Cheng;R. Nieberg;K. Lewin
研究了抗人乳脂球-2（HMFG-2）抗原的抗体，以评估其在检测胸水、心包液和腹腔液中肿瘤的价值。使用抗生物素蛋白-生物素复合物（ABC）技术评价了140份连续液体。比较常规细胞学和HMFG-2染色涂片，以前者作为肿瘤检测的标准。在15份标本中发现不一致结果（8例假阴性和7例假阳性）。两种方法之间差异的原因包括肿瘤细胞上缺乏HMFG-2抗原、随机抽样误差和子宫内膜细胞异位位于盆腔或在子宫器械中引入。我们得出结论，HMFG-2染色可能是一种有用的辅助技术，用于检测体液中的罕见肿瘤细胞，前提是HMFG-2阳性原发性肿瘤转移的可疑指数很高。
An antibody to human milk fat globule-2 (HMFG-2) antigen was investigated to assess its value in detecting tumors in pleural, pericardial and peritoneal fluids. One hundred forty consecutive fluids were evaluated using the avidin-biotin complex (ABC) technique. Conventional cytology and HMFG-2-stained smears were compared using the former as the standard for tumor detection. Discrepant results were found in 15 specimens (8 false negatives and 7 false positives). Causes for discrepancy between the methods included lack of HMFG-2 antigen on tumor cells, random sampling error and endometrial cells ectopically located in the pelvic cavity or introduced during uterine instrumentation. We conclude staining for HMFG-2 may be a useful adjuvant technique for the detection of rare tumor cells in body fluids provided there is a high index of suspicion of metastasis from an HMFG-2-positive primary neoplasm.