Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi
Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi
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DOI：
10.2307/3761610
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发表时间：
2001-01-01
期刊：
影响因子：
2.8
通讯作者：
Farrell, R
中科院分区：
文献类型：
--
作者：
Harrington, TC;McNew, D;Farrell, R
云杉蛇口菌复合体形成了昆虫分散的核菌纲单系类群，其中包括多线虫（Pesotum）和微线虫（Sporothrix）同源体。云杉复合体之外形成合丝状的其他蛇口菌物种缺乏孢子丝状态。云杉复合体中的九种已识别的物种由线状体形态、32°C 下的生长速率、交配反应和 rDNA 操纵子的内部转录间隔区 (ITS) 军团的序列来界定。 ITS 区域的系统发育分析表明该复合体中有两个主要分支，一个在主要针叶树寄主中引起蓝斑，另一个在主要硬木寄主上引起蓝斑。 III 针叶类群是O. piceae、O. canum、O. floccosum 和最近描述的O. setosum (anamorph Pesotum cupulatum sp. nov.)。硬木类群中有栎树 (O. querci)、卡托尼亚树 (O. catonianum) 和荷兰榆树病真菌：乌尔米 (O. ulmi)、新乌尔米 (O. novo-ulmi) 和希马尔-乌尔米 (O. Himal-ulmi)。 ITS 区域的限制性片段长度多态性被证明是界定这些物种的便捷诊断工具。
The Ophiostoma piceae complex forms a monophyletic group of insect-dispersed pyrenomycetes with synnemata (Pesotum) and micronematous (Sporothrix) synanamorphs. Other species of Ophiostoma outside of the O. piceae complex that form synnemata lack the Sporothrix state. The nine recognized species within the O. piceae complex are delimited by synnema morphology, growth rate at 32 C, mating reactions and sequences of the internal transcribed spacer (ITS) legion of the rDNA operon. Phylogenetic analysis of the ITS region suggests two major clades in the complex, one that causes bluestain in primarily coniferous hosts and the other on primarily hardwood hosts. III the coniferous group are O. piceae, O. canum, O. floccosum and the recently described O. setosum (anamorph Pesotum cupulatum sp. nov.). In the hardwood group are O. querci, O. catonianum, and the Dutch elm disease fungi: O. ulmi, O. novo-ulmi and O. himal-ulmi. Restriction fragment length polymorphisms of the ITS region are shown to be a convenient diagnostic tool for delimiting these species.