Investigation on the validity of 3D micro-CT imaging in the fish brain.
Investigation on the validity of 3D micro-CT imaging in the fish brain.
复制标题
鱼脑 3D 显微 CT 成像有效性的研究。
DOI：
10.1016/j.jneumeth.2019.108416
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发表时间：
2019
影响因子：
3
通讯作者：
Akihiro Takemura
中科院分区：
文献类型：
--
作者：
Shingo Udagawa;Keitaro Miyara;Hiroki Takekata;Yuki Takeuchi;Akihiro Takemura
背景微计算机断层扫描（Micro-computed tomography，CT）是一种非侵入性的技术，用于获得小动物组织结构的三维（3D）图像。与广泛的哺乳动物研究相比，很少有鱼类的三维成像研究已经使用micro-CT进行。一个优化的方法成像鱼的组织结构是必要的，因为他们已经适应了不同的环境，通过功能和结构specialization.New methodBrains的三个物种具有不同的大小和栖息地固定在4%的多聚甲醛和浸泡在非离子碘造影剂（碘帕铁）。我们研究碘帕铁浓度和浸泡时间之间的关系，以确定普遍适用于fish.ResultsWe最佳条件重建的3D图像的全鱼大脑的马拉巴尔石斑鱼（Epinealus malabaricus），杂种大比目鱼（Paralichthys olivaceus），和threespot濑鱼（Halichoeres trimaculatus）的大脑的横截面。在杂种大比目鱼中观察到脑结构的发育变化。三斑濑鱼的大部分大脑区域都是可以区分的，尽管大脑的内部区域不太明显。与现有方法的比较组织学技术通常用于观察鱼脑结构，尽管其在组织样本制备（收缩和变形）和图像方面存在缺陷。捕获（3D图像收缩）。在本研究中检查的技术解决了这些问题，并允许同时处理多个samples.ConclusionMicro-CT成像是适合观察各种鱼类的表面和内部结构。
BackgroundMicro-computed tomography (CT) is a non-invasive technique that is used to obtain three-dimensional (3D) images of tissue structure in small animals. Compared with extensive mammal studies, few 3D imaging studies of fish have been conducted using micro-CT. An optimized method for imaging fish tissue structure is necessary, because they have adapted to diverse environments via functional and structural specialization.New methodBrains of three species with different sizes and habitats were fixed in 4% paraformaldehyde and immersed in non-ionic iodinated contrast agent (Iopamiron). We examined the relationship between Iopamiron concentration and immersion time to determine universally optimal conditions for use in fish.ResultsWe reconstructed 3D images of whole fish brains from cross-sections of brains from the Malabar grouper (Epinephelus malabaricus), bastard halibut (Paralichthys olivaceus), and threespot wrasse (Halichoeres trimaculatus). Developmental changes in brain structure were observed in the bastard halibut. Most brain regions of the threespot wrasse were distinguishable, although inner regions of the brain were less visible.Comparison with existing methodsHistological techniques are typically used to observe fish brain structure, despite its drawbacks in terms of tissue sample preparation (shrinkage and distortion) and image capture (3D image constriction). The technique examined in the present study solves these problems and allows for the simultaneous handling of multiple specimens.ConclusionMicro-CT imaging is suitable for observing the surfaces and inner structures of fish of various species.