Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles
Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles
复制标题
DOI：
10.1128/jvi.00696-10
复制
发表时间：
2010-08-15
影响因子：
5.4
通讯作者：
Sittisombut, Nopporn
中科院分区：
文献类型：
--
作者：
Junjhon, Jiraphan;Edwards, Thomas J.;Sittisombut, Nopporn
在登革病毒复制期间，包膜糖蛋白prM的不完全切割产生成熟（prM-少）和含prM的未成熟细胞外颗粒的混合物。在这项研究中，连续免疫沉淀和冷冻电子显微镜揭示了第三种类型的细胞外颗粒，部分成熟的颗粒，作为主要的prM含颗粒在登革热血清型2病毒。pr-M连接突变体中病毒颗粒比例的变化显示prM切割水平的改变，表明部分成熟的颗粒可能代表病毒成熟途径中的中间亚群。这些发现与提示prM裂解的进行性模式的模型一致。
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.