PRODUCT INHIBITION OF BUTYRATE METABOLISM BY ACETATE AND HYDROGEN IN A THERMOPHILIC COCULTURE
PRODUCT INHIBITION OF BUTYRATE METABOLISM BY ACETATE AND HYDROGEN IN A THERMOPHILIC COCULTURE
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DOI：
10.1128/aem.54.10.2393-2397.1988
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发表时间：
1988-10-01
影响因子：
4.4
通讯作者：
WESTERMANN, P
中科院分区：
文献类型：
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作者：
AHRING, BK;WESTERMANN, P
对与嗜热自养甲烷杆菌（Methanobacterium thermoautotrophicum）互养联合的嗜热丁酸盐降解菌的产物抑制的研究表明，10-2大气压（2.03kPa）的氢气阻止生长和丁酸盐消耗，而1 × 104 kPa的较低氢气分压则阻止生长和丁酸盐消耗。10-3至2倍。10-2大气压（0.1 ~ 2.03kPa）逐渐抑制共培养物的丁酸消耗。在加入0.75 × 10 - 4时，没有发现丁酸盐消耗的抑制。10-3大气压（76 Pa）的氢气转化为气相。轻微的抑制丁酸消耗的共培养物发生在乙酸浓度为16.4 mM。抑制逐渐增加，随着乙酸浓度增加到81.4 mM，当完全抑制丁酸消耗发生。当培养物中除了M.在热自养条件下，随着乙酸浓度的降低，乙酸对产甲烷菌的抑制作用逐渐逆转。结果表明，丁酸盐高温降解菌的最佳生长条件取决于氢气和乙酸盐的去除。
Studies on product inhibition of a thermophilic butyrate-degrading bacterium in syntrophic association with Methanobacterium thermoautotrophicum showed that a gas phase containing more than 2 .times. 10-2 atm (2.03 kPa) of hydrogen prevented growth and butyrate consumption, while a lower hydrogen partial pressure of 1 .times. 10-3 to 2 .times. 10-2 atm (0.1 to 2.03 kPa) gradually inhibited the butyrate consumption of the coculture. No inhibition of butyrate consumption was found on the addition of 0.75 .times. 10-3 atm (76 Pa) of hydrogen to the gas phase. A slight inhibition of butyrate consumption by the coculture occurred at an acetate concentration of 16.4 mM. Inhibition gradually increased with increasing acetate concentration up to 81.4 mM, when complete inhibition of butyrate consumption occurred. When the culture contained an acetate-utilizing methanogen in addition to M. thermoautotrophicum, the inhibition of the triculture by acetate was gradually reversed as the acetate concentration was lowered by the aceticlastic methanogen. The results show that optimal growth conditions for the thermophilic butyrate-degrading bacterium depend on both hydrogen and acetate removal.