A FAMILY OF PUTATIVE POTASSIUM CHANNEL GENES IN DROSOPHILA
A FAMILY OF PUTATIVE POTASSIUM CHANNEL GENES IN DROSOPHILA
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DOI：
10.1126/science.2493160
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发表时间：
1989-02-17
期刊：
影响因子：
56.9
通讯作者：
SALKOFF, L
中科院分区：
文献类型：
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作者：
BUTLER, A;WEI, A;SALKOFF, L
Shaker钾通道编码基因被删除的突变果蝇仍然具有与Shaker基因编码的钾电流相似的钾电流。这表明果蝇中存在一个类似于shaker的基因家族。通过使用Shaker互补DNA探针和低强度杂交，现在已经分离出另外三个家族成员，Shab， Shaw和Shal。Shaker家族的基因并没有聚集在基因组中。Shab、Shaw和Shal蛋白与Shaker蛋白具有较高的同源性；整体膜部分的序列一致性大于50%。这些基因的组织与Shaker相似，因为每个信使RNA编码的只有一个同源结构域，其中包含所有电压门控离子通道共有的六个假定的跨膜片段。因此，钾通道的多样性可能来自于一个扩展的基因家族，以及Shaker初级转录物的交替剪接。
Mutant flies in which the gene coding for the Shaker potassium channel is deleted still have potassium currents similar to those coded by the Shaker gene. This suggests the presence of a family of Shaker-like genes in Drosophila. By using a Shaker complementary DNA probe and low-stringency hybridization, three additional family members have now been isolated, Shab, Shaw, and Shal. The Shaker family genes are not clustered in the genome. The deduced proteins of Shab, Shaw, and Shal have high homology to the Shaker protein; the sequence identity of the integral membrane portions is greater than 50 percent. These genes are organized similarly to Shaker in that only a single homology domain containing six presumed membrane-spanning segments common to all voltage-gated ion channels is coded by each messenger RNA. Thus, potassium channel diversity could result from an extended gene family, as well as from alternate splicing of the Shaker primary transcript.