Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro
Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro
复制标题
微粗糙化 TiO2/ZrO2 涂层的表征：体外机械性能和 HBMSC 响应
DOI：
10.1093/abbs/gmu040
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发表时间：
2014
影响因子：
3.7
通讯作者：
Shen Steve G. F.
中科院分区：
文献类型：
--
作者：
Si Jiawen;Zhang Jianjun;Liu Sha;Zhang Wenbin;Yu Dedong;Wang Xudong;Guo Lihe;Shen Steve G. F.
已有研究表明，采用ZrO 2作为第二相对生物陶瓷进行生物陶瓷化处理，可以显著提高等离子喷涂复合材料的结合强度。在本研究中，微粗糙二氧化钛/氧化锆（TiO 2/ZrO 2）（30重量% ZrO 2）涂层和TiO 2涂层等离子喷涂到钛板上。研究了两种涂层的显微组织特征和力学性能。比较了人骨髓间充质干细胞（HBMSCs）在TiO 2/ZrO 2和TiO 2涂层上的生物学行为和成骨分化能力。结果表明，TiO 2/ZrO 2涂层的剪切结合强度和显微硬度均显著高于TiO 2涂层。扫描电镜观察表明，与TiO 2涂层相比，TiO 2/ZrO 2涂层表面形成了更多的不规则突起和更密集的孔隙。进一步对比分析两种涂层上HBMSC增殖和成骨分化情况发现，与TiO 2涂层相比，在成骨培养后第10天，TiO 2/ZrO 2涂层上HBMSC的细胞碱性磷酸酶活性和Runx 2、Osterix表达水平显著升高，而细胞增殖和细胞外钙沉积无统计学显著差异。本研究表明，TiO 2/ZrO 2涂层可能有利于牙种植体的应用。
Previous studies have shown that using ZrO2as a second phase to bioceramics can significantly increase the bonding strength of plasma-sprayed composite material. In the present study, micro-roughened titanium dioxide/zirconia (TiO2/ZrO2) (30 wt% ZrO2) coating and TiO2coating were plasma-sprayed onto Ti plates. The micro-structural characteristics and mechanical properties of both coatings were investigated. Furthermore, the biological behavior and osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs) on both TiO2/ZrO2and TiO2coatings were compared. The results indicated that the shear bond strength and microhardness of TiO2/ZrO2coating were statistically higher than those of TiO2coating. Scanning electron microscope observation revealed that more irregularly shaped protuberances and denser pores were formed on the surface of TiO2/ZrO2coating compared with those of TiO2coating. Further comparative analysis of HBMSC proliferation and osteogenic differentiation on both coatings showed that significantly higher cellular alkaline phosphatase activity and expression levels of Runx2 and Osterix at day 10 after osteogenic culture were found on TiO2/ZrO2coating compared with TiO2coating, while no statistically significant difference in cell proliferation and extracellular calcium deposition was observed. The present study suggests that TiO2/ZrO2coating may be favorable for dental implant applications.