Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs
Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs
复制标题
DOI：
10.1261/rna.1738409
复制
发表时间：
2009-12-01
期刊：
影响因子：
4.5
通讯作者：
Hutvagner, Gyorgy
中科院分区：
文献类型：
--
作者：
Cole, Christian;Sobala, Andrew;Hutvagner, Gyorgy
深层测序技术，如Illumina、Solid和454平台，已经成为发现和量化不同生物体中的小RNA的非常强大的工具。对小RNA片段进行测序通常能识别出来自丰富的RNA物种的RNA，如rRNAs、tRNAs、SnRNA和snoRNA，它们被广泛认为是随机降解的产物。我们对深度测序的HeLa RNA进行了生物信息学分析，并经过质量过滤，鉴定出高度丰富的小RNA片段，这些片段来自成熟的tRNA，可能是通过特定的加工而不是随机降解产生的。此外，我们还证明了从tRNA(Gln)衍生的小RNA的加工在体内依赖于Disher，而Disher在体外切割tRNA。
Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become very powerful tools in discovering and quantifying small RNAs in diverse organisms. Sequencing small RNA fractions always identifies RNAs derived from abundant RNA species such as rRNAs, tRNAs, snRNA, and snoRNA, and they are widely considered to be random degradation products. We carried out bioinformatic analysis of deep sequenced HeLa RNA and after quality filtering, identified highly abundant small RNA fragments, derived from mature tRNAs that are likely produced by specific processing rather than from random degradation. Moreover, we showed that the processing of small RNAs derived from tRNA(Gln) is dependent on Dicer in vivo and that Dicer cleaves the tRNA in vitro.