Detection of occult cancer cells in peripheral blood and bone marrow by quantitative RT-PCR assay for cytokeratin-7 in breast cancer patients.
Detection of occult cancer cells in peripheral blood and bone marrow by quantitative RT-PCR assay for cytokeratin-7 in breast cancer patients.
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DOI：
10.3892/ijo.26.3.721
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发表时间：
2005-03
影响因子：
5.2
通讯作者：
Taka-aki Masuda;A. Kataoka;S. Ohno;S. Murakami;K. Mimori;T. Utsunomiya;H. Inoue;S. Tsutsui;J. Kinoshita;N. Masuda;N. Moriyama;M. Mori
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文献类型：
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作者：
Taka-aki Masuda;A. Kataoka;S. Ohno;S. Murakami;K. Mimori;T. Utsunomiya;H. Inoue;S. Tsutsui;J. Kinoshita;N. Masuda;N. Moriyama;M. Mori
隐匿性微转移（O.M）的临床意义尚不清楚。我们在外周血（P.B.）和骨髓（B.M.）乳腺癌手术患者中。首先，我们采用定量RT-PCR（QRT-PCR）技术检测了27种癌细胞系和8种非上皮细胞系中7种具有代表性的O.M分子标志物（CEA、CK-7、CK-18、CK-19、CK-20、MAM和MUC-1）的表达水平，结果显示CK-7在各癌细胞系中的表达水平均高于非上皮细胞系。其次，我们研究了O.M在P.B.中的临床意义。和B.M.之间。采用QRT-PCR方法检测乳腺癌手术患者血清CK-7水平。与17例非癌对照组比较，206例P.B.患者中CK-7阳性37例（18.0%），阳性100例（48.5%）。还有B.M.分别术后24个月以上观察98例，P. B.无病生存率（DFS）明显低于阴性组（P<0.01）。P. B.在132例淋巴结阴性病例中，CK-7阳性组的DFS比阴性组差（p=0.01），而且，在术后24个月观察的61例淋巴结阴性病例中，P. B.结果显示，与阴性对照组相比，阴性对照组的DFS较差（p<0.0001）。在BM，CK-7阳性组与CK-7阴性组间DFS无显著性差异。CK-7的QRT-PCR可作为O.M检测和PB中CK-7定量检测的一种有用且通用的方法作为乳腺癌手术患者早期复发的标志物具有预后价值。
The clinical significance of occult micrometastasis (O.M) remains unknown. We investigated it in peripheral blood (P.B.) and bone marrow (B.M.) in breast cancer patients with surgery. First, we investigated the expression levels of 7 representative molecular markers for detecting O.M (CEA, CK-7, CK-18, CK-19, CK-20, MAM and MUC-1) in 27 cancer and 8 non-epithelial cell lines using quantitative RT-PCR (QRT-PCR), and showed that the expression level of CK-7 was higher in every cancer cell line than in the non-epithelial cell lines. Next, we studied the clinical significance of O.M in P.B. and B.M. by QRT-PCR for CK-7 in breast cancer patients with surgery. Based on comparison with 17 non-cancer controls, 37 (18.0%) and 100 (48.5%) of the 206 patients were positive for CK-7 in P.B. and B.M., respectively. In 98 cases observed over 24 months after surgery, the CK-7-positive group in P.B. had poorer disease-free survival (DFS) than the negative group (p<0.01). The CK-7-positive group in P.B. showed poorer DFS than the negative group in 132 lymph node-negative cases (p=0.01), and moreover, in 61 lymph node-negative cases observed over 24 months after surgery, the CK-7-positive group in P.B. showed poorer DFS than the negative group (p<0.0001). In B.M., no significant difference in DFS was found between the CK-7-positive and CK-7-negative groups. QRT-PCR for CK-7 could be a useful and universal method for detecting O.M, and the quantitative detection of CK-7 in P.B. would have a prognostic value as a marker of early recurrence in breast cancer patients with surgery.