Detection of proteins on silica-silver core-shell substrates by surface-enhanced Raman spectroscopy.
Detection of proteins on silica-silver core-shell substrates by surface-enhanced Raman spectroscopy.
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DOI：
10.1016/j.jcis.2011.04.067
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发表时间：
2011-08
影响因子：
9.9
通讯作者：
Lei Chen;Xiaoxia Han;Jingxiu Yang;Ji Zhou;Wei Song;Bing Zhao;Weiqing Xu;Y. Ozaki
中科院分区：
文献类型：
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作者：
Lei Chen;Xiaoxia Han;Jingxiu Yang;Ji Zhou;Wei Song;Bing Zhao;Weiqing Xu;Y. Ozaki
我们已经采用了建议的二氧化硅-银核-壳（SSCS）SERS活性底物检测四个模型蛋白质：溶菌酶（一种蛋白质没有生色团），细胞色素（一种蛋白质与生色团的血红素），异硫氰酸荧光素（FITC）-抗人IgG（标记的FITC）和atto610-生物素/亲和素（识别标记的小分子）。这些蛋白质和SSCS基底上的拉曼标记的SERS光谱显示出高灵敏度和可重复性，这是由于电磁SERS增强以及修饰在SiO2纳米颗粒上的紧密堆积的Ag纳米颗粒内的额外定位场以及SiO2@Ag颗粒的聚集。我们发现吸附在SSCS基底上的atto 610-biotin/avidin的SERS强度比在Au修饰基底上镀Ag的SERS强度强约20倍。此外，所提出的基底的宽表面等离子体共振（SPR）将扩展SERS应用到更多的生物分子与不同的激光激发。
We have employed the proposed Silica–Silver Core–Shell (SSCS) SERS-active substrates to detect four model proteins: lysozyme (a protein without chromophore), cytochromec(a protein with chromophore of heme), fluorescein isothiocyanate (FITC)-anti human IgG (labeled with FITC) and atto610-biotin/avidin (recognition with labeled small molecules). SERS spectra of these proteins and Raman labels on the SSCS substrates show both high sensitivity and reproducibility, which are due to electromagnetic SERS enhancement with additional localization field within closely packed Ag nanoparticles decorated on the SiO2nanoparticles and the aggregation of SiO2@Ag particles. We have found that the SERS intensities of atto610-biotin/avidin adsorbed on the SSCS substrates are about 20 times stronger than those from Ag plating on Au-decorated substrate. Moreover, the broad surface plasmon resonance (SPR) of the proposed substrates will extend SERS applications to more biological molecules with different laser excitations.