One-step preparation of disposable multi-functionalized g-C3N4basedelectrochemiluminescence immunosensor for the detection of CA125
One-step preparation of disposable multi-functionalized g-C3N4basedelectrochemiluminescence immunosensor for the detection of CA125
复制标题
一次性制备用于检测CA125的一次性多功能g-C3N4电化学发光免疫传感器
DOI：
10.1016/j.snb.2015.11.133
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Xiaohua Jiang
中科院分区：
文献类型：
--
作者：
Lin Wu;Yuhong Sha;Wenrou Li;Sui Wang;Zhiyong Guo;Jun Zhou;Xiurong Su;Xiaohua Jiang
将多功能化石墨氮化碳（g-C3 N4）涂覆在一次性丝网印刷碳电极（SPCE）上，制备了一种新型的一次性无标记电化学发光（ECL）免疫传感器，用于检测肿瘤标志物糖类抗原125（CA 125）。用氨基包被的Fe 3 O 4纳米颗粒和CA 125抗体（抗CA 125）同时化学结合到羧基化的g-C3 N4的表面来制备多功能化的g-C3 N4。Fe 3 O 4纳米颗粒确保了ECL免疫传感器的外亥姆霍兹平面（OHP）中多功能化的g-C3 N4的存在，甚至成为电极表面的一部分。这种组装促进了g-C_3 N_4与电极之间的电子转移，从而大大增强了ECL强度。CA 125被抗CA 125选择性地捕获，导致ECL信号的有效抑制。在最佳条件下，该电化学发光免疫传感器对CA 125的检测线性范围为0.001 ~ 5 U/mL，检测下限为0.4 mU/mL，具有较高的灵敏度。此外，该免疫传感器还具有高特异性、良好的重现性和长期稳定性。所有这些特性为临床应用中快速、简单、选择性和有效地检测CA 125提供了极大的希望。
A novel, disposable and label-free electrochemiluminescence (ECL) immunosensor, based on multi-functionalized graphitic carbon nitride (g-C3N4) coated on the one-off screen-printed carbon electrodes (SPCEs), for the detection of tumor marker carbohydrate antigen 125 (CA125) was developed. The multi-functionalized g-C3N4 was prepared with amino-coated Fe3O4 nanoparticles and CA125 antibody (anti-CA125) chemically bound to the surface of carboxylated g-C3N4 simultaneously. The Fe3O4 nanoparticles ensured the presence of multi-functionalized g-C3N4 in the outer Helmholtz plane (OHP) of the ECL immunosensor and even becoming part of the electrode surface. This assembly promoted the electron transfer between g-C3N4 and the electrode resulting in greatly enhanced ECL intensity. The CA125 was selectively captured by anti-CA125 leading to effective inhibition of the ECL signal. Under optimal conditions, this disposable ECL immunosensor exhibited a wide dynamic linear detection range for CA125 (0.001-5 U/mL) with a lower detection limit of 0.4 mU/mL, providing high sensitivity. In addition, the immunosensor also exhibited high specificity, good reproducibility and long-term stability. All these properties offer great promise for rapid, simple, selective and effective detection of CA125 in clinical applications.