Regulation of muscular contractions in the human Fallopian tube through prostaglandins and progestagens
Regulation of muscular contractions in the human Fallopian tube through prostaglandins and progestagens
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DOI：
10.1093/humrep/den260
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发表时间：
2008-10-01
影响因子：
6.1
通讯作者：
Gemzell-Danielsson, K.
中科院分区：
文献类型：
--
作者：
Wanggren, K.;Stavreus-Evers, A.;Gemzell-Danielsson, K.
背景：配子和胚胎的运输是输卵管的重要功能。肌肉收缩和纤毛活动都与运输有关。前列腺素(PGs)是已知的肌肉收缩的介体。PG受体此前已在人类输卵管中被证实。目的：研究前列腺素和孕激素、抗孕激素、人绒毛膜促性腺激素和催产素对人输卵管肌肉收缩的影响，以及前列腺素受体的激素调节。切除输卵管壶腹-峡部连接处，用于体外收缩功能研究。研究了前列腺素E(1)、前列腺素E(2)、前列腺素F(2α)、孕酮、米非司酮、左旋诺孕酮、催产素和hCG对心肌收缩功能的影响。取输卵管组织培养24 h，应用免疫组织化学和实时荧光定量聚合酶链式反应技术研究孕激素和hCG对PG受体表达的影响。结果：经PGF(2α)和PGE(2)治疗后，肌肉收缩增加(P<；0.05)。经前列腺素E_1、孕酮、左旋诺孕酮、米非司酮、催产素和hCG治疗后，子宫收缩明显减少(P<；0.05)。输卵管组织中EP1、EP2、EP3和FP的相对信使核糖核酸表达增加(P<；0.05)。米非司酮和左炔诺孕酮治疗增加了EP1和EP2蛋白在管腔、肌肉和血管中的免疫染色强度。黄体酮和米非司酮增加血管内FP的免疫染色。结论：配子和胚胎的转运涉及PGs、孕酮、催产素和hCG对肌肉收缩的作用。
BACKGROUND: Transport of gametes and embryos is an important function of the Fallopian tube. Both muscular contractions and cilia activity are involved in the transportation. Prostaglandins (PGs) are known mediators of muscular contractility. PG receptors have previously been demonstrated in the human Fallopian tube. The aim was to study the effect of PGs and progestagens, antiprogestin, hCG and oxytocin on muscular contractions in the human Fallopian tube, and the hormonal regulation of PG receptors.METHODS: Twenty-two healthy women operated for benign causes were included in the study. The ampullary- isthmic junction of the Fallopian tubes was excised and used for in vitro contractility studies. The effect of PGE(1), PGE(2), PGF(2 alpha), progesterone, mifepristone, levonorgestrel, oxytocin and hCG on contractility was studied. Explants of Fallopian tubes were cultured for 24 h to study the effect of progestagens and hCG on the expression of PG receptors using immunohistochemistry and real- time PCR. RESULTS: Muscular contractions increased after treatment with PGF(2 alpha) and PGE(2) (P < 0.05). The contractions decreased after PGE1, progesterone, levonorgestrel, mifepristone, oxytocin and hCG ( P < 0.05). In tubal explant studies, relative mRNA expression of EP1, EP2, EP3 and FP increased after levonorgestrel treatment ( P < 0.05). Mifepristone and levonorgestrel treatment increased immunostaining intensity of EP1 and EP2 protein, in lumen, muscle and vessels. Progesterone and mifepristone increased immunostaining of FP in vessels. CONCLUSIONS: These data suggest that the transport of gametes and embryos involves the action of PGs, progesterone, oxytocin and hCG on muscular contractility.