Gap junction structures and distribution patterns of immunoreactive connexins 46 and 50 in lens regrowths of rhesus monkeys
Gap junction structures and distribution patterns of immunoreactive connexins 46 and 50 in lens regrowths of rhesus monkeys
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DOI：
10.1006/exer.1996.0021
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发表时间：
1996-02-01
影响因子：
3.4
通讯作者：
Tigges, M
中科院分区：
文献类型：
--
作者：
Lo, WK;Shaw, AP;Tigges, M
应用电镜和免疫荧光双标记技术研究了恒河猴正常晶状体和再生透镜中缝隙连接蛋白46（Cx46）和连接蛋白50（Cx 50）的分布特征。透镜再生物收集自幼年猴的无晶状体眼，其天然晶状体已在11-34个月前手术移除以模拟人类婴儿的单眼先天性白内障手术。大约90%的透镜再生物是位于虹膜后面的环形Soemmerring环。透镜再生由透镜上皮和包裹在肥大的囊材料内的透镜纤维组成。赤道区浅层多为形态正常的有核幼纤维，其他区多为肿胀纤维。在透镜再生过程中，正常和肿胀结构的纤维细胞之间容易观察到缝隙连接。在表面纤维，缝隙连接与细胞骨架成分无关。在中间和更深的皮质区域，肌动蛋白丝束被发现专门与间隙连接沿着他们的细胞质表面。免疫荧光双标记研究表明，在正常透镜的表层和深层皮质纤维中，Cx46和Cx 50在相同的缝隙连接中被标记。相反，在透镜再生中，仅在表面纤维中观察到Cx46和Cx 50的强共标记。Cx 50的标记是非常弱或在更深的皮质中不存在，而Cx46的强标记持续在整个更深的皮质的主要部分。Cx46的标记最终在更深的皮质中消失。本研究表明：（1）在正常晶状体中发现的肌动蛋白束/缝隙连接联合的分布模式在透镜再生中被观察到;（2）与正常晶状体相比，在恒河猴透镜再生中Cx 46和Cx 50的免疫反应性分布不同。(C)1996年学术出版社
Gap junction structures and distribution patterns of immunoreactive connexin46 (Cx46) and connexin 50 (Cx50) in normal lenses and lens regrowths of rhesus monkeys were studied using electron microscopy and immunofluorescence double-labeling. Lens regrowths were collected from aphakic eyes of young monkeys whose natural lenses had been surgically removed 11-34 months earlier to simulate monocular congenital cataract surgery in human infants. Approximately 90% of the lens regrowths examined was in the form of a doughnut-shaped Soemmerring's ring located behind the iris. The lens regrowth consisted of lens epithelium and lens fibers enclosed within hypertrophied capsular material. The superficial equatorial region usually contained nucleated young fibers of normal appearance, The other regions consisted of many swollen fibers. Gap junctions were readily observed between fiber cells of both normal and swollen configuration in the lens regrowth. In superficial fibers, gap junctions were not associated with cytoskeletal components. In the intermediate and the deeper cortical regions, actin filament bundles were found specifically associated with gap junctions along both of their cytoplasmic surfaces. An immunofluorescence double-labeling study showed that Cx46 and Cx50 were labeled in the same gap junctions in both superficial and deeper cortical fibers of the normal lens. In contrast, in the lens regrowth strong co-labeling of Cx46 and Cx50 was only observed in the superficial fibers. The labeling for Cx50 was very weak or absent in the deeper cortex, whereas the strong labeling for Cx46 persisted throughout the major portion of the deeper cortex. The labeling for Cx46 finally disappeared in the much deeper cortex. This study shows that (1) the same distribution pattern of actin bundle/gap junction association found in normal lenses is seen in the lens regrowth, and (2) the immunoreactive distribution of Cx46 and Cx50 differ in the lens regrowths as compared with those in the normal lenses of rhesus monieys. (C) 1996 Academic Press Limited