Evaluation of the attachment, proliferation, and differentiation of osteoblast on a calcium carbonate coating on titanium surface
Evaluation of the attachment, proliferation, and differentiation of osteoblast on a calcium carbonate coating on titanium surface
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钛表面碳酸钙涂层成骨细胞附着、增殖和分化的评价
DOI：
10.1016/j.msec.2011.03.003
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发表时间：
2011-07-20
影响因子：
7.9
通讯作者：
Wang, Yining
中科院分区：
文献类型：
--
作者：
Liu, Yi;Jiang, Tao;Wang, Yining
据报道，钛在牙科和骨科临床应用中存在一些局限性。本研究描述了一种使用简单化学方法在光滑钛（STi）和喷砂酸蚀钛（SATi）上制备碳酸钙涂层的涂层工艺，并评价了材料的体外生物学反应。STi、SATi、碳酸钙涂覆的STi（CC-STi）和碳酸钙涂覆的SATi（CC-SATi）的表面表征为表面粗糙度、接触角、表面形态和表面化学。扫描电镜和免疫荧光染色观察MG 63细胞在表面上的形态学变化。MTT法检测细胞贴壁增殖情况，碱性磷酸酶（ALP）活性检测细胞分化情况。发现MG 63有利地附着于碳酸钙晶体，在CC-STi和CC-SATi上具有较长的细胞质延伸，当分别与STi和SATi相比时，导致较低的细胞增殖但较高的ALP活性。此外，CC-SATi在生物学反应方面比CC-STi更有利。因此，钛表面的碳酸钙涂层可以促进骨整合过程，刺激成骨细胞的分化，尤其是在早期阶段。这种方法可能是未来临床应用的一种可行的替代选择。(C)2011 Elsevier B. V.保留所有权利。
Titanium has been reported to have some limitations in dental and orthopaedic clinical application. This study described a coating process using a simple chemical method to prepare calcium carbonate coatings on smooth titanium (STi) and sandblasted and acid-etched titanium (SATi), and evaluated the biological response of the materials in vitro. The surfaces of STi, SATi, calcium carbonate coated STi (CC-STi) and calcium carbonate coated SATi (CC-SATi) were characterized for surface roughness, contact angles, surface morphology and surface chemistry. The morphology of MG63 cells cultured on the surfaces was observed by SEM and Immuno-fluorescence staining. Cell attachment/proliferation was assessed by MTT assay, and cell differentiation was evaluated by alkaline phosphatase (ALP) activity. MG63 was found to attach favorably to calcium carbonate crystals with longer cytoplasmic extensions on CC-STi and CC-SATi, resulting in lower cell proliferation but higher ALP activity when compared to STi and SATi respectively. Moreover, CC-SATi is more favorable than CC-STi in terms of biological response. In conclusion, the calcium carbonate coatings on titanium were supposed to improve the osteointegration process and stimulate osteoblast differentiation, especially in early stage. And this method could possibly be a feasible alternative option for future clinical application. (C) 2011 Elsevier B.V. All rights reserved.