Isolation and characterization of rad51 orthologs from Coprinus cinereus and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs.
Isolation and characterization of rad51 orthologs from Coprinus cinereus and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs.
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灰盖鬼伞和番茄中 rad51 直向同源物的分离和表征，以及真核 reCA 同源物的系统发育分析。
DOI：
10.1007/s002940050189
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发表时间：
1997
期刊：
影响因子：
2.5
通讯作者：
Zolan,ME
中科院分区：
文献类型：
--
作者：
Stassen,NY;LogsdonJr,JM;Vora,GJ;Offenberg,HH;Palmer,JD;Zolan,ME
在真细菌中，该基因一直被认为是同源重组和DNA修复所必需的。最近的研究已经在古细菌和真核生物中发现了该基因的同源物，从而强调了该基因在DNA代谢中的普遍作用。我们从担子菌cinereus和被子植物lycopersicon esculentum中分离并鉴定了两个新的同源物。与酿酒酵母的therad51基因一样，该基因在γ辐射和减数分裂过程中受到高度诱导。真核生物同源基因的系统发育分析揭示了真核生物进化早期的基因重复，产生了两个推测的单系类群。其中一组共有11个特征基因，命名为therad51group，与糖酵母菌rad51基因同源，还包含葡萄球菌和番茄红蛋白基因。另一组由7个基因组成，称为dmc1组，与糖酵母菌的dmc1基因同源。序列比较和系统发育分析揭示了RecA蛋白进化速率的广泛谱系和基因特异性差异。Dmc1一直比Rad51进化得更快，两种类型的真菌蛋白，尤其是酵母菌的真菌蛋白，变化很快，特别是与缓慢进化的脊椎动物蛋白相比。drosophilarad51蛋白经历了非常快速的序列分化。
In eubacteria, therecAgene has long been recognized as essential for homologous recombination and DNA repair. Recent work has identifiedrecAhomologs in archaebacteria and eukaryotes, thus emphasizing the universal role this gene plays in DNA metabolism. We have isolated and characterized two newrecAhomologs, one from the basidiomyceteCoprinus cinereusand the other from the angiospermLycopersicon esculentum. Like theRAD51gene ofSaccharomyces cerevisiae, theCoprinusgene is highly induced by gamma irradiation and during meiosis. Phylogenetic analyses of eukaroticrecAhomologs reveal a gene duplication early in eukaryotic evolution which gave rise to two putatively monophyletic groups ofrecA-like genes. One group of 11 characterized genes, designated therad51group, is orthologous to theSaccharomyces RAD51gene and also contains theCoprinusandLycopersicongenes. The other group of seven genes, designated thedmc1group, is orthologous to theSaccharomyces DMC1gene. Sequence comparisons and phylogenetic analysis reveal extensive lineage- and gene-specific differences in rates of RecA protein evolution. Dmc1 consistently evolves faster than Rad51, and fungal proteins of both types, especially those ofSaccharomyces, change rapidly, particularly in comparison to the slowly evolving vertebrate proteins. TheDrosophilaRad51 protein has undergone remarkably rapid sequence divergence.