ISOLATION OF A NOVEL-38 RESIDUE-HYPOTHALAMIC POLYPEPTIDE WHICH STIMULATES ADENYLATE-CYCLASE IN PITUITARY-CELLS
ISOLATION OF A NOVEL-38 RESIDUE-HYPOTHALAMIC POLYPEPTIDE WHICH STIMULATES ADENYLATE-CYCLASE IN PITUITARY-CELLS
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DOI：
10.1016/0006-291x(89)91757-9
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发表时间：
1989-10-16
影响因子：
3.1
通讯作者：
COY, DH
中科院分区：
文献类型：
--
作者：
MIYATA, A;ARIMURA, A;COY, DH
从绵羊下丘脑组织中分离到一种能刺激大鼠垂体前叶细胞腺苷酸环化酶的神经肽。其氨基酸序列为：His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln- Met-Ala-Val-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2。N-末端序列[1-28]显示与血管活性肠多肽（VIP）的同源性为68%，但腺苷酸环化酶刺激活性至少是VIP的1000倍。在10 ~（-10）M（GH，PRL，ACTH）或10 ~（-9）M（LH）的剂量下，它能增加灌流的大鼠垂体细胞生长激素（GH）、催乳素（PRL）、促肾上腺皮质激素（ACTH）和促黄体生成素（LH）的释放。这些促垂体作用是否是该肽的主要作用，或者垂体中的哪些生理作用与该肽对腺苷酸环化酶的刺激有关，仍有待确定。
A novel neuropeptide which stimulates adenylate cyclase in rat anterior pituitary cell cultures was isolated from ovine hypothalamic tissues. Its amino acid sequence was revealed as: His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln- Met-Ala-Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Arg-Val-Lys-Asn-Lys-NH2. The N-terminal sequence [1-28] shows 68% homology with vasoactive intestinal polypeptide (VIP) but is adenylate cyclase stimulating activity was at least 1000 times greater than that of VIP. It increased release of growth hormone (GH), prolactin (PRL), corticotropin (ACTH) and luteinizing hormone (LH) from superfused rat pituitary cells at as small a dose as 10-10M (GH, PRL, ACTH) or 10-9M (LH). Whether these hypophysiotropic effects are the primary actions of the peptide or what physiological action in the pituitary is linked with the stimulation of adenylate cyclase by this peptide remains to be determined.