Characteristics of transplantable tumors induced in the rat by N-2-fluorenylacetamide: elevations in tissue and serum sialic acid.
Characteristics of transplantable tumors induced in the rat by N-2-fluorenylacetamide: elevations in tissue and serum sialic acid.
复制标题
N-2-芴基乙酰胺在大鼠中诱导的可移植肿瘤的特征：组织和血清唾液酸升高。
DOI：
10.1093/jnci/64.6.1401
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
D. Morré
中科院分区：
文献类型：
--
作者：
T. M. Kloppel;D. Morré
肿瘤和血清中唾液酸和含唾液酸的糖脂（神经节苷脂）水平与肿瘤生长特性之间的相关性在可移植肝癌和鳞状细胞癌中进行了研究，这些肝癌和鳞状细胞癌是由致癌物N-2-芴基乙酰胺引发的，并在体内和组织培养中繁殖。肿瘤细胞系在组织学分类、生长速率和形成肺转移的能力方面各不相同。生长速度和组织学分类之间以及这两个参数和转移能力之间均无相关性。总唾液酸和神经节苷脂唾液酸水平升高，在致癌剂治疗的肝脏和移植性肝癌时，与正常肝脏相比。唾液酸水平与肝癌的生长速度呈弱相关。非转移性肝癌细胞系的神经节苷脂表现出较少的N-乙酰神经氨酸-半乳糖-葡萄糖-N-酰基鞘氨醇（GM 3）和总的单唾液酸神经节苷脂的比例增加，双唾液酸神经节苷脂比转移性细胞系。转移性肝癌细胞系的神经节苷脂模式比非转移性细胞系更接近正常肝脏的神经节苷脂模式。在皮下植入动物的血清中观察到总唾液酸和神经节苷脂唾液酸水平同时升高。血清总唾液酸水平确实与肿瘤组织中发现的总唾液酸水平相关。血清唾液酸水平与血清唾液酸转移酶活性水平无直接相关性。组织和血清神经节苷脂唾液酸的升高是N-2-芴乙酰胺给药后大鼠肝脏肿瘤发生的一致特征。此外，它们似乎是与肿瘤细胞分化或转移不直接相关的早期事件。
The correlation between levels of sialic acid and sialic acid-containing glycolipids (gangliosides) in tumors and serum with the growth characteristics of the tumors was investigated in transplantable hepatomas and squamous cell carcinomas initiated with the carcinogen N-2-fluorenylacetamide and propagated in vivo and in tissue culture. Tumor lines varied in histologic classification, growth rate, and ability to form pulmonary metastases. There was neither a correlation between growth rate and histologic classification nor between either of these two parameters and the ability to metastasize. Total and ganglioside sialic acid levels were elevated in carcinogen-treated liver and in transplantable hepatomas when contrasted with normal liver. Levels of sialic acid showed a weak correlation with the growth rate of hepatomas. Gangliosides from nonmetastatic hepatoma lines exhibited less N-acetylneuraminic acid--galactose--glucose-N--acylsphingosine (GM3) and an increased ratio of total monosialogangliosides to disialogangliosides than did metastatic lines. Ganglioside patterns of metastatic hepatoma lines more closely resembled the ganglioside patterns of normal liver than did those of the nonmetastatic lines. Concomitant elevations of total and ganglioside sialic acid levels were observed in sera of animals bearing subcutaneous implants. Serum levels of total sialic acid did correlate with total sialic acid levels found in the tumor tissues. The levels of serum sialic acid were not correlated directly with levels of serum sialyltransferase activity. Elevations of both tissue and serum ganglioside sialic acid were consistent features of liver tumorigenesis in the rat after N-2-fluorenylacetamide administration. They appeared, furthermore, to be early events not directly related to tumor cell differentiation or metastasis.