EFFECT OF BILATERAL NEPHRECTOMY ON PRODUCTION AND DISTRIBUTION OF MURAMIDASE (LYSOZYME) IN RAT
EFFECT OF BILATERAL NEPHRECTOMY ON PRODUCTION AND DISTRIBUTION OF MURAMIDASE (LYSOZYME) IN RAT
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DOI：
10.1139/y70-021
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发表时间：
1970-01-01
影响因子：
2.1
通讯作者：
KEELER, R
中科院分区：
文献类型：
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作者：
KEELER, R
双侧肾切除术后大鼠全身胞壁质酶增加50%以上。平均增加率为每 100g 340μg/h。皮肤和骨骼的增幅最大。尽管肺部含有高水平的胞壁质酶，但肾切除术后酶活性水平没有显着变化。输尿管切片或输尿管静脉吻合的大鼠并未出现高血浆胞壁质酶水平。因此，对肾切除术的反应不是对尿毒症的反应。用可的松或DL-乙硫氨酸预处理动物对肾切除术的酶反应没有影响。这表明反应不是针对抗原物质并且不依赖于酶的肝脏合成。用细胞毒剂环磷酰胺预处理降低了胞壁酶的血浆和全身水平，并阻断了对肾切除术的反应。直接测量表明骨髓产生胞壁质酶的速率大幅增加。由于无法在体外证明肾组织对胞壁质酶的灭活作用，因此得出的结论是，肾切除术导致酶产生率增加，而不是分解代谢失败。肾组织通常可能对胞壁酶的形成产生抑制作用。
After bilateral nephrectomy total body muramidase in the rat increased by more than 50%. The mean rate of increase was 340 μg/h per 100 g. Skin and bone showed the greatest increase. Although the lungs contain a high level of muramidase there was no significant change in the level of enzyme activity after nephrectomy. Rats with sectioned ureters or with a uretero–venous anastomosis did not develop high plasma levels of muramidase. The response to nephrectomy is therefore not a response to uremia. Pretreatment of the animals with cortisone orDL-ethionine had no effect on the enzyme response to nephrectomy. This was taken to indicate that the response was not to antigenic material and did not depend upon hepatic synthesis of the enzyme. Pretreatment with the cytotoxic agent cyclophosphamide reduced the plasma and total body level of muramidase and blocked the response to nephrectomy. Direct measurement demonstrated a large increase in the rate of muramidase production by bone marrow. Since the in vitro inactivation of muramidase by kidney tissue could not be demonstrated, it is concluded that nephrectomy causes an increase in the rate of enzyme production rather than a failure of catabolism. Renal tissue might normally exert an inhibitory influence upon muramidase formation.