Determining the role of Rab7 in constitutive and ligand-mediated epidermal growth factor receptor endocytic trafficking using single cell assays.
Determining the role of Rab7 in constitutive and ligand-mediated epidermal growth factor receptor endocytic trafficking using single cell assays.
复制标题
使用单细胞测定确定 Rab7 在组成型和配体介导的表皮生长因子受体内吞运输中的作用。
DOI：
10.1007/978-1-4939-2569-8_26
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ceresa,BrianP
中科院分区：
文献类型：
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作者：
Ceresa,BrianP
RAB proteins are essential for the proper membrane trafficking of a number of proteins. Each of the 60+ RABs that have been identified has a discrete role in coordinating the movement from one subcellular compartment to another. Early attempts at deciphering the roles of individual RAB proteins relied heavily on the use of activating and/or dominant negative mutants (Ceresa, Histol Histopathol 21:987–993, 2006). However, overexpression of mutant proteins can lead to misleading information; high levels of expression can drive low affinity (and possibly, nonphysiological) interactions as well as cause mislocalization. The use of RNAi for transient protein knock down will reveal which membrane trafficking steps absolutely require the attenuated RAB. When determining the role of RAB protein in epidermal growth factor receptor (EGFR) membrane trafficking, there are special considerations. The EGFR undergoes constitutive and ligand-mediated endocytic trafficking. Both affect receptor signaling, but via different mechanisms. Here, we discuss how to experimentally dissect those two processes.
通过内体保留调节表皮生长因子受体的内吞后运输。
影响因子：
4.8
作者：
J. Herbst;L. Opresko;B. Walsh;Douglas A. Lauffenburger;H. Wiley
通讯作者：
H. Wiley
DOI：
10.1073/pnas.80.5.1337
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KAWAMOTO, T;SATO, JD;MENDELSOHN, J
通讯作者：
MENDELSOHN, J