Prolactin inhibition of pregnant mare's serum stimulated follicle development in the rat ovary.
Prolactin inhibition of pregnant mare's serum stimulated follicle development in the rat ovary.
复制标题
孕马血清的催乳素抑制刺激了大鼠卵巢中的卵泡发育。
DOI：
10.1080/07435809009107117
复制
发表时间：
1990
影响因子：
2.1
通讯作者：
Odell,WD
中科院分区：
文献类型：
--
作者：
Larsen,JL;Bhanu,A;Odell,WD
催乳素（PRL）对女性生殖系统的影响被认为主要发生在下丘脑-垂体水平。以下研究旨在评估PRL是否可以直接改变卵巢水平的促性腺激素作用。第一个实验，10组7只周期成年雌性大鼠，每天两次给予妊娠母马血清（PMS: 25 IU）和生理盐水（SAL）或PRL(0.25、0.8、2.5、8或25ug)，连续4天。在第二个实验中，所有动物都给予PMS（6个剂量：0-75 IU/d， 16只动物/剂量），每个PMS剂量的动物中有一半接受PRL （25ug，每天两次），一半接受等体积的稀释剂。最后，垂体切除（hypox）成年大鼠（n=5-6/组）接受25 IU PMS/d和PRL (0-75 ug)，每日2次。另一组给予0次PRL和0次PMS。在每项研究结束时评估卵巢重量和组织学。在第一个实验中，PRL以剂量依赖的方式抑制pms刺激的卵巢体重增加（p<0.01）。与对照组相比，PRL抑制剂量（25ug BID）小鼠的窦前卵泡数量（p<0.005）和窦前卵泡数量（p<0.05）均减少。在第二个实验中，PRL （25 ug BID）再次抑制经前综合症刺激的卵巢重量（p<0.01）。最后，在低氧动物中，与对照组相比，PRL抑制pms刺激的卵巢增重（25和75ug PRL: p=0.001），以及卵巢窦泡（8-75ug PRL）的平均数量（p<0.001）和直径（p<0.001）。综上所述，PRL可抑制经前综合症刺激的卵巢增重，以及成年雌性大鼠的窦腔卵泡直径（仅限低氧动物）和数量，这表明在高泌乳素血症状态下，PRL除了下丘脑和垂体作用外，还可直接在卵巢水平改变促性腺激素介导的活性（即卵泡生成）。
Prolactin (PRL) effects on the female reproductive system have been presumed to occur primarily at the hypothalamic-pituitary level. The following studies were designed to evaluate whether PRL can directly alter gonadotropin actions at the ovarian level. In the first experiment, 10 groups of 7 cycling adult female rats were given a daily dose of pregnant mare's serum (PMS: 25 IU) and either saline (SAL) or PRL (0.25, 0.8, 2.5, 8, or 25ug) twice daily for 4 days. In the second experiment, PMS (6 doses: 0-75 IU/d; 16 animals/dose) was administered to all animals while half the animals at each PMS dose received PRL (25ug twice daily) and half received an equal volume of diluent. Finally, hypophysectomized (hypox) adult rats (n=5-6/group) received 25 IU PMS/d and PRL (0-75 ug) twice daily. An additional group re-ceived 0 PRL and 0 PMS. Ovarian weight and histology were evaluated at the completion of each study. In the first experiment, PRL inhibited PMS-stimulated ovarian weight gain in a dose-dependent manner (p<0.01). Numbers of preantral (p<0.005) and antral (p<0.05) follicles were decreased in animals receiving an inhibitory dose of PRL (25ug BID) compared to controls. In the second experiment PRL (25 ug BID) again inhibited PMS-stimulated ovarian weight (p<0.01) at all doses of PMS. Finally, in hypox animals, PRL inhibited PMS-stimulated ovarian weight gain (25 and 75 ug PRL: p=0.001), and mean number (p<0.001) and diameter (p<0.001) of antral ovarian follicles (8-75ug PRL) compared to controls. In summary, administration of PRL inhibited PMS-stimulated ovarian weight gain, and antral follicle diameter (in hypox animals only) and number in adult female rats suggesting that in states of hyperprolactinemia, PRL alters gonadotropin-mediated activities (i.e., folliculogenesis) directly at the ovarian level in addition to its hypothalamic and pituitary actions.