Macrophage podosomes assemble at the leading lamella by growth and fragmentation
Macrophage podosomes assemble at the leading lamella by growth and fragmentation
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DOI：
10.1083/jcb.200212037
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发表时间：
2003-05-26
影响因子：
7.8
通讯作者：
Matsudaira, P
中科院分区：
文献类型：
--
作者：
Evans, JG;Correia, I;Matsudaira, P
足小体是巨噬细胞前缘含有肌动蛋白和纤维蛋白的黏附物。在转染了β -肌动蛋白- ecfp和l -纤维蛋白- eyfp的细胞中，足质体组装的定量四维显微镜显示，在细胞周围产生新的粘附是通过两种机制之一；新生足体组装，或前体足体分裂成子足体。大的足小体簇前体似乎也是一种粘附结构；它含有肌动蛋白、纤维蛋白、整合素，并与基质密切相关。微管抑制剂紫杉醇和去焦碱抑制足小体的周转和极化形成，但不影响这些结构中肌动蛋白的周转率。由于子足小体和足小体簇前体优先位于前沿，它们可能在不断产生新的细胞粘附位点中发挥关键作用。
Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with beta-actin-ECFP and L-fimbrin-EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosome cluster precursor also appears to be an adhesion structure; it contains actin, fimbrin, integrin, and is in close apposition to the substratum. Microtubule inhibitors paclitaxel and demecolcine inhibit the turnover and polarized formation of podosomes, but not the turnover rate of actin in these structures. Because daughter podosomes and podosome cluster precursors are preferentially located at the leading edge, they may play a critical role in continually generating new sites of cell adhesion.