Direct observation of orientation distributions of actin filaments in a solution undergoing shear banding
Direct observation of orientation distributions of actin filaments in a solution undergoing shear banding
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直接观察剪切带溶液中肌动蛋白丝的取向分布
DOI：
10.1039/c6sm02832b
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发表时间：
2017
期刊：
影响因子：
3.4
通讯作者：
T. Nakagaki and H. Orihara
中科院分区：
文献类型：
--
作者：
K. Sato;I. Kunita;Y. Takikawa;D. Takeuchi;Y. Tanaka;T. Nakagaki and H. Orihara
剪切带是复杂流体中常见的现象。然而，大分子在溶液中经历剪切带的配置尚未被直接观察到。在这项研究中，通过使用的事实，F-肌动蛋白溶液表现出剪切带和肌动蛋白丝可视化的荧光标记，我们直接观察到的内在状态的肌动蛋白溶液进行剪切带。通过结合标记的肌动蛋白丝的三维成像和粒子图像测速技术（PIV），我们得到了肌动蛋白丝在高剪切速率和低剪切速率区域的取向分布，并指出了它们的定量差异。此外，通过使用的取向分布和应用棒状聚合物的应力表达式，我们估计在高和低剪切速率区域的应力张量。这一评价表明，不同取向分布的丝状大分子可以表现出共同的剪切应力。
Shear banding is frequently observed in complex fluids. However, the configuration of macromolecules in solutions undergoing shear banding has not yet been directly observed. In this study, by using the fact that F-actin solutions exhibit shear banding and actin filaments are visualized by fluorescent labels, we directly observed the intrinsic states of an actin solution undergoing shear banding. By combining the 3D imaging of labeled actin filaments and particle image velocimetry (PIV), we obtained orientation distributions of actin filaments in both high and low shear rate regions, whose quantitative differences are indicated. In addition, by using the orientation distributions and applying stress expression for rod-like polymers, we estimated stress tensors in both high and low shear rate regions. This evaluation indicates that different orientation distributions of filamentous macromolecules can exhibit a common shear stress.