Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus
Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus
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氯霉素抑制稻瘟菌真核丝氨酸/苏氨酸磷酸酶和感染特异性细胞分化
DOI：
10.1038/s41598-019-41039-x
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发表时间：
2019
影响因子：
4.6
通讯作者：
Kamakura Takashi
中科院分区：
文献类型：
--
作者：
Nozaka Akihito;Nishiwaki Ayaka;Nagashima Yuka;Endo Shogo;Kuroki Misa;Nakajima Masahiro;Narukawa Megumi;Kamisuki Shinji;Arazoe Takayuki;Taguchi Hayao;Sugawara Fumio;Kamakura Takashi
氯霉素（Chloramphenicol，Cm）是一种广谱、经典的抗原核生物抗生素。然而，Cm在真核生物中具有严重的副作用，其原因仍然未知。稻瘟病菌是一种引起水稻稻瘟病的植物病原真菌，它通过单细胞分化形成附着胞侵染寄主细胞。氯霉素特异性地抑制附着胞的形成，这表明Cm在稻瘟病菌中具有新的分子靶标。应用T7噬菌体展示方法推测，Ser/Thr蛋白磷酸酶MoDullard可能是Cm的靶点。在动物中，Dullard在细胞分化和蛋白质合成中起作用，但在真菌中，其作用知之甚少。体内和体外分析表明，MoDullard是附着胞形成所必需的，Cm可以结合并抑制MoDullard功能。鉴于人磷酸酶CTDSP 1在M.因此，CTDSP 1可能是Cm在真核生物中的一个新的分子靶点。
Chloramphenicol (Cm) is a broad-spectrum classic antibiotic active against prokaryotic organisms. However, Cm has severe side effects in eukaryotes of which the cause remains unknown. The plant pathogenic fungusMagnaporthe oryzae, which causes rice blast, forms an appressorium to infect the host cell via single-cell differentiation. Chloramphenicol specifically inhibits appressorium formation, which indicates that Cm has a novel molecular target (or targets) in the rice blast fungus. Application of the T7 phage display method inferred that MoDullard, a Ser/Thr-protein phosphatase, may be a target of Cm. In animals Dullard functions in cell differentiation and protein synthesis, but in fungi its role is poorly understood.In vivoandin vitroanalyses showed that MoDullard is required for appressorium formation, and that Cm can bind to and inhibit MoDullard function. Given that human phosphatase CTDSP1 complemented the MoDullard function during appressorium formation byM. oryzae, CTDSP1 may be a novel molecular target of Cm in eukaryotes.