The role of polyamines, Na(+) and K(+) in the formation of triple helices between purine oligonucleotides and the promoter region of the human c-src proto-oncogene.
The role of polyamines, Na(+) and K(+) in the formation of triple helices between purine oligonucleotides and the promoter region of the human c-src proto-oncogene.
复制标题
多胺、Na( ) 和 K( ) 在嘌呤寡核苷酸和人 c-src 原癌基因启动子区域之间形成三螺旋中的作用。
DOI：
10.1093/nar/28.12.2307
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发表时间：
2000
影响因子：
14.9
通讯作者：
Lee,JS
中科院分区：
文献类型：
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作者：
Aich,P;Thomas,TJ;Lee,JS
在多胺、Na+和K+存在下，用荧光偏振法测定了富含嘌呤的寡核苷酸与人c-src原癌基因的嘧啶·嘌呤段形成三链体的结合常数。在六胺和四胺系列中，较长的多胺在低浓度的多胺下具有较大的三链体形成结合常数。在较高浓度下，所有值均趋于稳定在109/M范围内。与以前的报道相反，K+不抑制三链体的形成，并且在150 mM下，11聚体和22聚体寡核苷酸的结合常数再次在109/M范围内。在150 mM K+下，多胺的加入没有导致结合常数的任何显着增加。经测定，K+抑制的缺乏是由于荧光偏振技术所需的嘌呤寡核苷酸浓度低（1 nM）。在较高浓度（1 µM）下，观察到寡核苷酸的自缔合。这些结果表明，在体内，至少对于c-src启动子，由K+抑制三链体的形成可能不是有害的。然而，即使在聚阳离子存在下，也可能难以实现高于~109/M的结合常数。
Binding constants for triplex formation between purine-rich oligonucleotides and a pyrimidine·purine tract of the human c-srcproto-oncogene were measured by fluorescence polarization in the presence of polyamines, Na+and K+. In both the hexamine and tetramine series, the longer polyamines had the larger binding constants for triplex formation at low concentrations of polyamine. At higher concentrations all values tended to plateau in the 109/M range. In contrast to previous reports, K+did not inhibit triplex formation and at 150 mM the binding constants were again in the 109/M range for both an 11mer and 22mer oligonucleotide. At 150 mM K+the addition of polyamines did not lead to any significant increase in the binding constants. It was determined that the lack of inhibition by K+was due to the low concentration (1 nM) of purine oligonucleotide required for the fluorescence polarization technique. At higher concentrations (1 µM) self-association of the oligonucleotide was observed. These results suggest thatin vivo, at least for the c-srcpromoter, the inhibition of triplex formation by K+may not be detrimental. However, it may be difficult to achieve binding constants above ~109/M even in the presence of polycations.
在精胺的四胺和五胺类似物存在下，嘧啶-嘌呤-嘧啶三链体 DNA 稳定化。
DOI：
--
发表时间：
1997
期刊：
Biochemistry and cell biology = Biochimie et biologie cellulaire.
影响因子：
--
作者：
Thomas,TJ;Ashley,C;Thomas,T;Shirahata,A;Sigal,LH;Lee,JS
通讯作者：
Lee,JS