Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
复制标题
DOI：
10.1590/s1517-83822013005000022
复制
发表时间：
2013-01-01
影响因子：
2.2
通讯作者：
Mota, Rinaldo Aparecido
中科院分区：
文献类型：
--
作者：
Silva, Karla Patrícia Chaves da;Takaki, Galba Maria de Campos;Mota, Rinaldo Aparecido
为了评估巴西生产的PPD-Mallein的效力，五只动物来自一处被确定为腺体聚集地的财产。这些动物有提示疾病的临床症状，另外五只，来自无腺体的特性，没有表现出临床症状和血清学阴性(对照组)。以三氯乙酸和硫酸铵为沉淀剂，从马来伯克霍尔德氏菌中提取PPD-顺丁烯二醛。根据农业、畜牧和供应部(MAPA)制定的腺体诊断标准对动物进行接种。应用PPD-Mallein 48h后，用药动物出现局部肿胀、眼部分泌物和泪液。对照组接种PPD-Mallein后未见炎症反应。这种在巴西生产并仍在测试中的免疫原可以有效地识别真阳性动物的感染，排除真阴性动物的感染，为腺体的诊断和控制提供了一种新的可能性。
To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichloroacetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no inflammatory reaction at the site of inoculation of PPD-mallein. This immunogen produced in Brazil and still being tested was effective for identifying the infection in true positive animals and excluding the truly negative ones, being a new possibility for diagnosis and control of glanders