Elimination of Electrochemically-Active Interferents in Amperometric Biosensor Measurements; Application to On-Line Monitoring of Glucose and Glutamate
Elimination of Electrochemically-Active Interferents in Amperometric Biosensor Measurements; Application to On-Line Monitoring of Glucose and Glutamate
复制标题
消除电流生物传感器测量中的电化学活性干扰物；
DOI：
10.1080/00032719308019868
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发表时间：
1993
影响因子：
2
通讯作者：
L. Symon
中科院分区：
文献类型：
--
作者：
A. Koshy;E. Zilkha;T. Obrenovitch;H. P. Bennetto;D. Richards;L. Symon
摘要介绍了一种消除电化学活性物质干扰的新方法。样品溶液通过插入样品流线的铂管电极，在那里进行直流预电解，然后输送到含有酶电极的检测池。当使用安培酶流动细胞分析脑外液中的谷氨酸、葡萄糖或乳酸时，这种方法是专门为避免单胺和抗坏血酸的干扰而开发的。反应产物的HPLC分析表明，预氧化处理有效地去除了主要干扰物，但对目标分析物没有影响。
Abstract A novel method of eliminating interferences which arise from electrochemically active substances is described. The sample solutions are passed through a platinum tube electrode inserted into the sample flow line, where they are subjected to DC pre-electrolysis before delivery to a detector cell containing an enzyme electrode. This method was specifically developed to avoid the interference of monoamines and ascorbic acid when brain extracellular fluid is analysed for glutamate, glucose or lactate using an amperometric enzyme flow-cell. HPLC analysis of the reaction products showed that the pre-oxidation treatment efficiently removes the principal interferents, but leaves unaffected the analyte of interest.