Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate
Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate
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DOI：
10.1007/s00299-008-0583-0
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发表时间：
2008-10-01
影响因子：
6.2
通讯作者：
Tsutsumi, Nobuhiro
中科院分区：
文献类型：
--
作者：
Fujimoto, Masaru;Arimura, Shin-ichi;Tsutsumi, Nobuhiro
拟南芥基因组有六个动力相关蛋白家族。这些家族之一包括 DRP2A 和 DRP2B。该家族蛋白质的结构域与动物内吞蛋白动力蛋白的结构域最相似。在本研究中，在拟南芥根尖细胞和烟草培养细胞的细胞板中强烈检测到 GFP 标记的 DRP2B 信号。对烟草培养细胞胞质分裂过程中这些信号的延时观察表明，DRP2B 主要定位于细胞板新形成的部分，并且 DRP2B 的定位动态与 DRP1A 非常相似，DRP1A 是一种拟南芥动力相关蛋白，与大豆成膜蛋白密切相关。这些结果表明，来自两个不同家族的拟南芥动力相关蛋白 DRP1A 和 DRP2B 在细胞板中相似的位置参与膜重塑。
The Arabidopsis genome has six families of dynamin-related proteins. One of these families includes DRP2A and DRP2B. The domain structures of proteins of this family are most similar to those of the animal endocytosis protein, dynamin. In this study, the signals of GFP-tagged DRP2B were strongly detected in the cell plate of Arabidopsis root tip cells and tobacco cultured cells. Time-lapse observations of these signals during cytokinesis in tobacco cultured cells suggested that DRP2B mainly localized to the newly formed part of the cell plate, and that the localization dynamics of DRP2B was quite similar to that of DRP1A, which is an Arabidopsis dynamin-related protein that is closely related to soybean phragmoplastin. These results indicate that Arabidopsis dynamin-related proteins, DRP1A and DRP2B, from two different families, participate in membrane remodeling at a similar place in the cell plate.