Evaluation of Adipose-Derived Stromal Vascular Fraction or Bone Marrow-Derived Mesenchymal Stem Cells for Treatment of Osteoarthritis
Evaluation of Adipose-Derived Stromal Vascular Fraction or Bone Marrow-Derived Mesenchymal Stem Cells for Treatment of Osteoarthritis
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DOI：
10.1002/jor.20933
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Mcllwraith, C. Wayne
中科院分区：
文献类型：
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作者：
Frisbie, David D.;Kisiday, John D.;Mcllwraith, C. Wayne
本研究的目的是评估关节内施用脂肪源性基质血管成分或骨髓源性间充质干细胞治疗骨关节炎的临床、生化和组织学效果。通过关节镜在所有马的中腕关节中诱导骨关节炎，对侧关节进行假手术。所有马都在第 14 天接受治疗。八匹马接受安慰剂治疗，八匹马在受骨关节炎影响的关节中接受脂肪源性基质血管部分。最后八匹马接受了骨髓间充质干细胞治疗骨关节炎影响的关节。评估包括临床、放射照相、滑液分析、总体、组织学、组织化学和生化评估。没有观察到与治疗相关的不良事件。该模型引起了除两个参数之外的所有参数的显着变化，没有证明显着的治疗效果，除了与安慰剂相比，骨髓源性间充质干细胞的滑液渗出物 PGE2 水平有所改善。与脂肪源性基质血管成分和安慰剂治疗相比，骨髓源性间充质干细胞的改善更大。总的来说，这项研究的结果不足以推荐使用干细胞来治疗该模型中代表的骨关节炎。 (C) 骨科研究会。由 Wiley periodicals, Inc. 出版。J Orthop Res 27:1675-1680, 2009
The purpose of this study was the assessment of clinical, biochemical, and histologic effects of intraarticular administered adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. Osteoarthritis was induced arthroscopically in the middle carpal joint of all horses, the contralateral joint being sham-operated. All horses received treatment on Day 14. Eight horses received placebo treatment and eight horses received adipose-derived stromal vascular fraction in their osteoarthritis-affected joint. The final eight horses were treated the in osteoarthritis-affected joint with bone marrow-derived mesenchymal stem cells. Evaluations included clinical, radiographic, synovial fluid analysis, gross, histologic, histochemical, and biochemical evaluations. No adverse treatment-related events were observed. The model induced a significant change in all but two parameters, no significant treatment effects were demonstrated, with the exception of improvement in synovial fluid effusion PGE2 levels with bone marrow-derived mesenchymal stem cells when compared to placebo. A greater improvement was seen with bone marrow-derived mesenchymal stern cells when compared to adipose-derived stromal vascular fraction and placebo treatment. Overall, the findings of this study were not significant enough to recommend the use of stern cells for the treatment of osteoarthritis represented in this model. (C) Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1675-1680, 2009