Medaka vasa is required for migration but not survival of primordial germ cells
Medaka vasa is required for migration but not survival of primordial germ cells
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DOI：
10.1016/j.mod.2009.02.004
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发表时间：
2009-05-01
影响因子：
2.6
通讯作者：
Hong, Yunhan
中科院分区：
文献类型：
--
作者：
Li, Mingyou;Hong, Ni;Hong, Yunhan
Vasa对生殖细胞的发育至关重要。然而，在不同的动物门中，维管所涉及的精确过程有很大的不同。在这里，我们表明，血管是所需的原始生殖细胞（PGC）在青鳉鱼迁移。两个吗啉代的vasa敲低导致PGC迁移缺陷，通过共注射Vasa RNA来挽救。有趣的是，Vasa敲除并没有改变PGC的数量，身份，增殖和运动性，即使在异位位置。我们建立了一个体外单细胞水平追踪PGCs的细胞培养体系。在此培养体系中，对照组和注射吗啉的原肠胚产生相同的PGC数量和相同的PGC存活时间。重要的是，培养物中vasa耗尽的PGCs具有与正常PGCs相似的运动性和运动性。迁移组织中wt1a、sdf1b和cxcT4b的表达模式在Vasa敲低后保持不变。通过嵌合体的形成，我们发现，从输精管耗尽囊胚PGCs未能迁移正常的环境中，而控制PGCs迁移正常输精管破裂的胚胎。此外，在输精管耗尽胚胎异位PGC也保留了所有的PGC属性检查。总之，青鳉血管是PGC迁移所需的细胞自主性，但对PGC增殖，运动，身份和存活是不利的。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Vasa is essential for germline development. However, the precise processes in which vasa involves vary considerably in diverse animal phyla. Here we show that vasa is required for primordial germ cell (PGC) migration in the medakafish. vasa knockdown by two morpholinos led to the PGC migration defect that was rescued by coinjection of Vasa RNA. Interestingly, Vasa knockdown did not alter the PGC number, identity, proliferation and motility even at ectopic locations. We established a cell culture system for tracing PGCs at the single cell level in vitro. In this culture system, control and morpholino-injected gastrulae produced the same PGC number and the same time course of PGC survival. importantly, vasa-depleted PGCs in culture had similar motility and locomotion to normal PGCs. Expression patterns of wt1a, sdf1b and cxcT4b in migratory tissues remained unchanged by Vasa knockdown. By chimera formation we show that PGCs from vasa-depleted blastulae failed to migrate properly in the normal environment, whereas control PGCs migrated normally in vasa-disrupted embryos. Furthermore, ectopic PGCs in vasa-depleted embryos also retained all the PGC properties examined. Taken together, medaka vasa is cell-autonomously required for PGC migration, but dispensable to PGC proliferation, motility, identity and survival. (C) 2009 Elsevier Ireland Ltd. All rights reserved.