SYNTHESIS OF HYALURONIC-ACID IS DECREASED AND SYNTHESIS OF PROTEOGLYCANS IS INCREASED WHEN CULTURED MOUSE EPIDERMAL-CELLS DIFFERENTIATE
SYNTHESIS OF HYALURONIC-ACID IS DECREASED AND SYNTHESIS OF PROTEOGLYCANS IS INCREASED WHEN CULTURED MOUSE EPIDERMAL-CELLS DIFFERENTIATE
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DOI：
10.1111/1523-1747.ep12275707
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发表时间：
1986-06-01
影响因子：
6.5
通讯作者：
HASCALL, VC
中科院分区：
文献类型：
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作者：
LAMBERG, SI;YUSPA, SH;HASCALL, VC
新生小鼠表皮细胞在0.05 mM Ca 2+培养基中培养时增殖，当Ca 2+增加至约1.2 mM（大多数细胞培养基中的水平）时终末分化。我们发现，透明质酸和蛋白多糖的合成分离培养的新生小鼠表皮细胞和这些大分子的量和质的变化时，出现增殖表皮培养物诱导分化钙。一个主要的变化，发生分化是减少合成的透明质酸，而合成的蛋白多糖和糖蛋白增加。在这些培养物中合成的蛋白聚糖是硫酸乙酰肝素-蛋白聚糖（90%）和硫酸软骨素-蛋白聚糖（10%），与钙水平无关。
Newborn mouse epidermal cells proliferate when cultured in 0.05 mM Ca2+ medium and terminally differentiate when the Ca2+ is increased to about 1.2 mM, the level found in most cell culture media. We found that hyaluronic acid and proteoglycans were synthesized by isolated cultured newborn mouse epidermal cells and that quantitative and qualitative changes in these macromolecules appeared when proliferating epidermal cultures were induced to differentiate by calcium. A major change that occurred with differentiation was a reduction in synthesis of hyaluronic acid while synthesis of proteoglycans and glycoproteins increased. The proteoglycans synthesized in these cultures were heparan sulfate-proteoglycan (90%) and chondroitin sulfate-proteoglycan (10%), regardless of the calcium level.