7-Oxoorobanchyl Acetate and 7-Oxoorobanchol as Germination Stimulants for Root Parasitic Plants from Flax (Linum usitatissimum)
7-Oxoorobanchyl Acetate and 7-Oxoorobanchol as Germination Stimulants for Root Parasitic Plants from Flax (Linum usitatissimum)
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DOI：
10.1271/bbb.90021
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发表时间：
2009-06
期刊：
影响因子：
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通讯作者：
Xiaonan Xie;K. Yoneyama;J. Kurita;Y. Harada;Yoichi M. A. Yamada;Y. Takeuchi;K. Yoneyama
中科院分区：
文献类型：
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作者：
Xiaonan Xie;K. Yoneyama;J. Kurita;Y. Harada;Yoichi M. A. Yamada;Y. Takeuchi;K. Yoneyama
对亚麻（Linum usitatissimum L.）根寄生植物的发芽刺激剂进行了纯化和鉴定。亚麻根分泌物中含有至少8种有效成分，液相色谱-串联质谱（LC-MS /MS）和气相色谱-质谱（GC-MS）分析表明，其中含有6种独角酯内酯。通过与合成标准品的NMR、GC-MS和LC-MS /MS数据比较，鉴定出其中2个化合物为邻苯二甲酸乙酯和邻苯二甲酸乙酯。通过一维和二维NMR、ESI -和EI-MS分析，对其中一种新型三聚己内酯进行了纯化，并确定为7-氧基乙酸乙酯[(（3aS,4S,8bS，E)-8,8-二甲基-3-（(R)-4-甲基-5-氧-2,5-二氢呋喃-2-氧基）亚甲基）-2,7-二氧基-3,3a,4,5,6,7,8,8 -八氢- 2h -吲哚[1,2-b]呋喃-4-乙酸酯]。另一种也经过纯化，鉴定为7-氧基溴凤尾鱼酚。其余两种化合物由于稀缺性而无法表征。
Germination stimulants for root parasitic plants produced by flax (Linum usitatissimum L.) were purified and characterized. The root exudate of flax contained at least 8 active fractions, and liquid chromatography-tandem mass spectrometry (LC–MS/MS) and gas chromatography mass spectrometry (GC–MS) analyses suggested that there were 6 strigolactones. Two of them were identified as orobanchol and orobanchyl acetate by comparing NMR and GC–MS and LC–MS/MS data with those of synthetic standards. One of the two novel strigolactones was purified and determined as 7-oxoorobanchyl acetate [((3aS,4S,8bS,E)-8,8-dimethyl-3-(((R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-2,7-dioxo-3,3a,4,5,6,7,8,8b-octahydro-2H-indeno[1,2-b]furan-4-yl acetate) by 1D and 2D NMR spectroscopic, and ESI– and EI–MS spectrometric analyses. The other one was also purified and identified as 7-oxoorobanchol. The remaining two compounds could not been characterized due to their scarcity.