A Method for Recording Evoked Local Field Potentials in the Primate Dentate Gyrus In Vivo
A Method for Recording Evoked Local Field Potentials in the Primate Dentate Gyrus In Vivo
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DOI：
10.1002/hipo.20773
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发表时间：
2011-05-01
期刊：
影响因子：
3.5
通讯作者：
Ono, Taketoshi
中科院分区：
文献类型：
--
作者：
Tamura, Ryoi;Eifuku, Satoshi;Ono, Taketoshi
在活体海马中记录诱发的局部场电位（LFPs）为我们提供了关于学习和记忆的神经机制的有用信息。虽然这项技术已被用于啮齿类动物、兔形目动物和猫科动物的海马体研究，但尚未应用于灵长类动物的海马体。在这里，我们报告的方法记录诱发LFPs在猴子的海马。在电生理记录的指导下，将刺激电极和记录电极分别植入穿支通路和齿状回。当刺激强度略高于阈值时，fEPSP出现一个缓慢的正波成分，即场兴奋性突触后电位（fEPSP）;随着刺激强度的增加，fEPSP的幅值增加，随后出现一个尖锐的负波成分，即群体锋电位。当记录或刺激电极的坐标被逐步移动时，我们观察到诱发LFP的波形发生系统性变化;这种变化对应于电极通过的结构布置。在成对脉冲刺激的短期突触可塑性测试中，第二脉冲诱发的电位以依赖于脉冲间间隔的方式受到第一脉冲的影响。在通过高频刺激进行的长时程突触可塑性测试中，fEPSP的斜率和群体尖峰的面积增加超过1小时。这些结果表明，在本研究中开发的方法是有用的灵长类动物海马功能的理论测试。(C)2010 Wiley-Liss，Inc.
Recording evoked local field potentials (LFPs) in the hippocampus in vivo has yielded us useful information about the neural mechanisms of learning and memory. Although this technique has been used in studies of the hippocampus of rodents, lagomorphs, and felines, it has not yet been applied to the primate hippocampus. Here, we report a method for recording evoked LFPs in the hippocampus of monkeys. A stimulation electrode and a recording electrode were implanted in the perforant pathway and dentate gyrus, respectively, under the guidance of electrophysiological recording. With a low stimulus intensity just above the threshold, the potential appeared as a slow positive-wave component, which was regarded as field excitatory postsynaptic potential (putative fEPSP); as stimulation intensity increased, the fEPSP amplitude increased, followed by a sharp negative component which was regarded as putative population spike. When the coordinates of the recording or stimulation electrode were moved stepwise, we observed a systematic change in the waveforms of evoked LFPs; this change corresponded to the structural arrangement through which the electrode passed. In a test for short-term synaptic plasticity by paired-pulse stimulation, potentials evoked by the second pulse were influenced by the first one in a manner dependent on interpulse intervals. In a test for long-term synaptic plasticity by high-frequency stimulation, the slopes of the fEPSPs and the area of population spikes were increased for more than 1 h. These results indicate that the method developed in the present study is useful for testing theories of hippocampal functions in primates. (C) 2010 Wiley-Liss, Inc.