Bu-Shen-Ning-Xin Decoction ameliorated the osteoporotic phenotype of ovariectomized mice without affecting the serum estrogen concentration or uterus.
Bu-Shen-Ning-Xin Decoction ameliorated the osteoporotic phenotype of ovariectomized mice without affecting the serum estrogen concentration or uterus.
复制标题
补肾宁心汤改善去势小鼠骨质疏松表型，且不影响血清雌激素浓度或子宫
DOI：
10.2147/dddt.s89505
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Li DJ
中科院分区：
文献类型：
--
作者：
Wang L;Qiu XM;Gui YY;Xu YP;Gober HJ;Li DJ
补肾宁心汤（BSNXD）是一种治疗绝经后骨质疏松症的中药复方，但其对骨代谢和子宫的影响尚未见报道。目的观察补肾活血汤对去卵巢小鼠骨骼和子宫的影响，评价补肾活血汤的有效性和安全性。材料与方法采用去势法建立绝经后骨质疏松症动物模型，用补肾活血汤治疗。采用双能X线骨密度仪测定骨密度，采用组织形态计量学方法测定骨代谢相关参数。进行钙黄绿素标记以检测骨形成。取小鼠子宫称重，进行组织形态计量学分析。制备药物衍生血清，通过酶免疫测定法评估17-β-雌二醇浓度。结果BSNXD给药可改善OVX小鼠的骨质疏松表型，表现为骨密度和骨体积的增加;芳香化酶抑制剂来曲唑的给药不能消除这些作用。BSNXD对血清雌激素浓度及子宫无明显影响。结论补肾活血汤对去卵巢小鼠外周血雌激素水平及子宫无明显影响，对雌激素缺乏性骨丢失有一定的改善作用。
Introduction Bu-Shen-Ning-Xin Decoction (BSNXD), a traditional Chinese medicinal composition, has been used as a remedy for postmenopausal osteoporosis, but its effects on bone metabolism and the uterus have not been reported. Purpose We aimed to determine the respective effects of BSNXD on the bones and the uterus of ovariectomized (OVX) mice to evaluate the efficacy and safety of this herbal formula. Materials and methods Postmenopausal osteoporosis animal models that were generated by ovariectomy were treated with BSNXD. Dual-energy X-ray absorptiometry was performed to analyze the bone mineral density, and histomorphometric analysis was performed to measure the parameters related to bone metabolism. Calcein labeling was performed to detect bone formation. The uteruses from the mice were weighed, and the histomorphometry was analyzed. Drug-derived serum was prepared to assess the 17-β-estradiol concentration via enzyme immunoassay. Results BSNXD administration ameliorated the osteoporotic phenotype of OVX mice, as evidenced by an increase in the bone mineral density and bone volume; these effects could not be abolished by the administration of the aromatase inhibitor letrozole. Moreover, BSNXD had no effect on the serum estrogen concentration or uterus. Conclusion These results suggest that BSNXD has ameliorating effects on bone loss due to estrogen deprivation without affecting the peripheral blood estrogen concentration or the uterus in OVX mice.