Three genes, IgtF, Iic2C and IpsA, have a primary role in determining the pattern of oligosaccharide extension from the inner core of Haemophilus influenzae LPS
Three genes, IgtF, Iic2C and IpsA, have a primary role in determining the pattern of oligosaccharide extension from the inner core of Haemophilus influenzae LPS
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DOI：
10.1099/mic.0.26912-0
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发表时间：
2004-07-01
期刊：
影响因子：
2.8
通讯作者：
Moxon, ER
中科院分区：
文献类型：
--
作者：
Hood, DW;Deadman, ME;Moxon, ER
脂多糖（LPS）是流感嗜血杆菌的毒力决定因子，并且在菌株内和菌株之间表现出实质性的结构异质性。导致这种异质性的关键因素是将第一个葡萄糖添加到LPS内核的三个庚糖残基中的每一个所需的基因。在每种情况下，这种添加可以促进进一步的寡糖延伸。IgtF总是存在于菌株中，并且产物具有将葡萄糖添加到第一庚糖的功能。Iic2C存在于一半的菌株中，并且发现将葡萄糖添加到第二庚糖。将Iic2C插入到不天然含有它的菌株中导致从LPS的第二庚糖掺入己糖。IpsA基因的产物可以将葡萄糖或半乳糖添加到第三庚糖。通过菌株之间IpsA的等位基因替换，表明基因的序列可以是这种特异性的唯一决定因素。因此，IgtF、Iic2C和IpsA对H.流感病毒LPS。
Lipopolysaccharide (LPS) is a virulence determinant of Haemophilus influenzae and exhibits substantial heterogeneity in structure within and between strains. Key factors contributing to this heterogeneity are the genes required to add the first glycose to each of the three heptose residues of the LPS inner core. In each case this addition can facilitate further oligosaccharide extension. IgtF is invariably present in strains and the product has a function in adding the glucose to the first heptose. Iic2C is present in half the strains and was found to add a glucose to the second heptose. Insertion of Iic2C into a strain that does not naturally contain it resulted in hexose incorporation from the second heptose of the LPS. The product of the IpsA gene can add a glucose or galactose to the third heptose. By allelic replacement of IpsA between strains it is shown that the sequence of the gene can be the sole determinant of this specificity. Thus, IgtF, Iic2C and IpsA make significant but very distinct contributions to the conservation and variable patterns of oligosaccharide extensions seen in H. influenzae LPS.