The evolutionary portrait of metazoan NAD salvage.
The evolutionary portrait of metazoan NAD salvage.
复制标题
DOI：
10.1371/journal.pone.0064674
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Silva RM
中科院分区：
文献类型：
--
作者：
Carneiro J;Duarte-Pereira S;Azevedo L;Castro LF;Aguiar P;Moreira IS;Amorim A;Silva RM
烟酰胺腺嘌呤二核苷酸（NAD）水平对细胞稳态和生存至关重要。细胞内NAD的主要来源是来自烟酰胺的挽救途径，其中烟酰胺磷酸核糖基转移酶（NAMPTs）和烟酰胺酶（pnc）起关键作用。NAMPTs和pnc在衰老、感染和糖尿病和癌症等疾病条件中很重要。这些酶被认为是多余的，因为其中一种或另一种存在于每个个体的基因组中。NAMPT和PNC的共存是最近才在无脊椎动物中发现的，但没有结构或功能表征。通过表达和进化分析，结合同源性建模和蛋白配体对接，我们发现这两个基因在主要无脊椎动物分支的关键物种中同时表达，并强调了后动物NAMPT和PNC同源物的序列和结构守恒模式。研究结果预测，NAMPTs和pnc同时活跃，提高了NAD回收途径不是多余的可能性，因为在某些物种中，这两种途径都是为了满足NAD生产的需要而维持的。
Nicotinamide Adenine Dinucleotide (NAD) levels are essential for cellular homeostasis and survival. Main sources of intracellular NAD are the salvage pathways from nicotinamide, where Nicotinamide phosphoribosyltransferases (NAMPTs) and Nicotinamidases (PNCs) have a key role. NAMPTs and PNCs are important in aging, infection and disease conditions such as diabetes and cancer. These enzymes have been considered redundant since either one or the other exists in each individual genome. The co-occurrence of NAMPT and PNC was only recently detected in invertebrates though no structural or functional characterization exists for them. Here, using expression and evolutionary analysis combined with homology modeling and protein-ligand docking, we show that both genes are expressed simultaneously in key species of major invertebrate branches and emphasize sequence and structural conservation patterns in metazoan NAMPT and PNC homologues. The results anticipate that NAMPTs and PNCs are simultaneously active, raising the possibility that NAD salvage pathways are not redundant as both are maintained to fulfill the requirement for NAD production in some species.
登录
查看更多内容
影响因子：
64.8
作者：
Burnett, Camilla;Valentini, Sara;Cabreiro, Filipe;Goss, Martin;Somogyvari, Milan;Piper, Matthew D.;Hoddinott, Matthew;Sutphin, George L.;Leko, Vid;McElwee, Joshua J.;Vazquez-Manrique, Rafael P.;Orfila, Anne-Marie;Ackerman, Daniel;Au, Catherine;Vinti, Giovanna;Riesen, Michele;Howard, Ken;Neri, Christian;Bedalov, Antonio;Kaeberlein, Matt;Soti, Csaba;Partridge, Linda;Gems, David
通讯作者：
Gems, David
影响因子：
2.9
作者：
French, Jarrod B.;Cen, Yana;Vrablik, Tracy L.;Xu, Ping;Allen, Eleanor;Hanna-Rose, Wendy;Sauve, Anthony A.
通讯作者：
Sauve, Anthony A.
影响因子：
4.8
作者：
Balan, Vitaly;Miller, Gregory S.;Tzivion, Guri
通讯作者：
Tzivion, Guri
影响因子：
3.9
作者：
Hu, Gang;Taylor, Alexander B.;Hart, P. John
通讯作者：
Hart, P. John
影响因子：
56.9
作者：
Domergue, R;Castaño, I;Cormack, BP
通讯作者：
Cormack, BP