High-performance amperometric biosensors and biofuel cell based on chitosan-strengthened cast thin films of chemically synthesized catecholamine polymers with glucose oxidase effectively entrapped.
High-performance amperometric biosensors and biofuel cell based on chitosan-strengthened cast thin films of chemically synthesized catecholamine polymers with glucose oxidase effectively entrapped.
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DOI：
10.1016/j.bios.2010.09.058
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发表时间：
2011-01
影响因子：
12.6
通讯作者：
Chao Chen;Lihua Wang;Yueming Tan;Cong Qin;Fangyun Xie;Yingchun Fu;Q. Xie;Jinhua Chen;S. Yao
中科院分区：
文献类型：
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作者：
Chao Chen;Lihua Wang;Yueming Tan;Cong Qin;Fangyun Xie;Yingchun Fu;Q. Xie;Jinhua Chen;S. Yao
利用K3 Fe（CN）6快速氧化多巴胺（DA）或去甲肾上腺素（NA），得到葡萄糖氧化酶（GOx）包埋的聚多巴胺（PDAC）或聚去甲肾上腺素（PNAC），并将这种包埋酶的聚合物浇铸在Au电极上，然后用壳聚糖（CS）强化，用于生物传感和生物燃料电池（BFC）的制备.优化后的CS/PDAC-GOx/Au葡萄糖生物传感器具有极高的灵敏度（高达135μAmM−1cm-2）、极低的检测限（0.07μM）、<3s的响应时间、良好的干扰抑制、惊人的热稳定性（在60°C下寿命为3周，30°C下寿命超过2个月）和高的抗尿素变性能力。该生物传感器在以对苯醌（BQ）或二茂铁一元羧酸（Fc）为人工介质的第二代生物传感模式下也能很好地工作，其线性检测范围大大拓宽（BQ为2.0μM-48.0mM，Fc为2.0μM-16.0mM），葡萄糖饱和电流密度高达mAcm−2级。人工介质穿过酶膜的良好渗透性使得能够基于所报道的动力学模型定量固定化GOx的表面浓度，并且使用UV-Vis分光光度法测量酶活性，揭示CS/PDAC-GOx/Au的高酶活性/负载。以CS/PDAC-GOx/Au为阳极，在含有100 mM葡萄糖和4.0 mM BQ的磷酸盐缓冲溶液中，以Nafion膜隔离的酸性KMnO 4为碳阴极，成功地制备了BFC，其最大功率密度为1.62 mWcm − 2，优于迄今报道的大多数BFC的功率密度，为上级。
Rapid oxidation of dopamine (DA) or l-noradrenaline (NA) by K3Fe(CN)6yields poly(DA) (PDAC) or poly(NA) (PNAC) with glucose oxidase (GOx) effectively entrapped, and such an enzyme-entrapped catecholamine polymer is cast on an Au electrode followed by chitosan (CS) strengthening for biosensing and fabrication of a biofuel cell (BFC). The optimized glucose biosensor of CS/PDAC–GOx/Au displays an extremely high sensitivity up to 135μAmM−1cm–2, a very low limit of detection of 0.07μM, a response time of <3s, good suppression of interferents, striking thermostability (lifetime of 3 weeks at 60°C and over 2 months at 30°C), and high resistance to urea denaturation. The biosensor also works well in the second generation biosensing mode with p-benzoquinone (BQ) or ferrocene monocarboxylic acid (Fc) as an artificial mediator, with greatly broadened linear detection ranges (2.0μM–48.0mM for BQ and 2.0μM–16.0mM for Fc) and up to mAcm−2-scale glucose-saturated current density. The good permeability of artificial mediators across the enzyme film enables the quantification of the surface concentration of immobilized GOx on the basis of a reported kinetic model, and UV–Vis spectrophotometry is used to measure the enzymatic activity, revealing high enzymatic activity/load at CS/PDAC–GOx/Au. A BFC is also successfully fabricated with a bioanode of CS/PDAC–GOx/Au in phosphate buffer solution containing 100mM glucose and 4.0mM BQ and a carbon cathode in Nafion-membrane-isolated acidic KMnO4, and its maximum power density of 1.62mWcm−2is superior to those of most BFC hitherto reported.