On‐column injection onto capillary columns. Part 2: Study of sampling conditions; practical recommendations
On‐column injection onto capillary columns. Part 2: Study of sampling conditions; practical recommendations
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毛细管柱的柱上进样第 2 部分：采样条件研究；
DOI：
10.1002/jhrc.1240010510
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
K. Grob
中科院分区：
文献类型：
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作者：
K. Grob
在一年的连续使用的柱上注射，在我们的第一份报告中描述的典型优势已得到充分证实。此外，对大量样品的分析已证明是有前途的。仅对柱上进样器装置进行了微小修改。已经收集了广泛的证据，表明只有当冷捕集或溶剂效应作为谱带缩短机制起作用时，柱上注射后毛细管柱的完全分离效率才能达到。虽然在柱上注射的条件下，冷捕集的效率低于其他注射技术，但溶剂效应的情况正好相反。与不分流进样相比，增加了溶剂在柱上过度冷凝的危险。提出了一个工作规则，以建立最佳的色谱条件，处理大的样品体积，同时确保充分的分离效率，但避免损害柱。
During one year continuous use of on-column injection, the typical advantages described in our first report have fully been confirmed. In addition the analysis of large sample volumes has proved promising. Only minor modifications have been applied to the on-column injector device. Broad evidence has been gathered showing that full separation efficiency of the capillary columns after on-column injection is attained only when cold trapping or the solvent effect, as band shortening mechanisms, are working- While, under the conditions of on-column injection, cold trapping is less efficient than with other injection techniques, the opposite holds true for the solvent effect. Compared with splitless injection, the danger of excessive solvent condensation on the column is increased. A working rule is presented for establishing the optimal chromatographic conditions for handling large sample volumes while ensuring full separation efficiency yet avoiding harm to the column.