Phospholipid fatty acyl spatial distribution in bovine rod outer segment disk membranes
Phospholipid fatty acyl spatial distribution in bovine rod outer segment disk membranes
复制标题
DOI：
10.1016/s0005-2736(97)00200-9
复制
发表时间：
1998-01-05
影响因子：
3.4
通讯作者：
Paw, Z
中科院分区：
文献类型：
--
作者：
Albert, AD;Young, JE;Paw, Z
研究了脂肪酸在磷脂头群类中的分布与牛棒外节盘膜年龄/空间分布的关系。这些圆盘根据其膜中的胆固醇含量被分成亚群。由于盘片膜胆固醇含量随着盘片在杆外段顶端移位而降低，这种分离产生了不同年龄的盘片亚群，并且来自与年龄相关的外段空间位置。对各亚群的磷脂、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）和磷脂酰肌醇（PI）进行了分离，并测定了各亚群的脂肪酸组成。这些数据表明，虽然大多数脂肪酸随着年龄/空间位置的变化很少或没有变化，但在某些类别中可以观察到一些明显的变化。在PC类中，16:0随着磁盘使用时间的增加而急剧减少，而22:6随着磁盘使用时间的增加而增加。虽然体育课脂肪酸有一些变化，但变化不大。PS类的脂肪酸组成无显著变化。占总磷脂不到2%的PI类显示出与年龄相关的变化，每种脂肪酸都可以测量。其中最值得注意的是，当椎间盘顶端移位时，20:4的比例增加。这些变化表明盘状膜的重塑可能与光导过程或为最终的盘状吞噬做准备有关。(C) 1998爱思唯尔科学有限公司
The distribution of fatty acids within the phospholipid headgroup classes was investigated as a function of the age/spatial distribution of bovine rod outer segment disk membranes. The disks were separated into subpopulations based upon the cholesterol content in their membranes. Because disk membrane cholesterol content decreases as the disks are apically displaced in the rod outer segment, this separation yields disk subpopulations of different ages and from age-dependent spatial locations within the outer segment. The phospholipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI), of each of these subpopulations were separated and the fatty acid composition of each was determined. These data indicated that while most of the fatty acids show little or no change with age/spatial location, some pronounced changes can be observed in certain classes. Within the PC class, 16:0 dramatically decreases with disk age while the 22:6 increases with disk age. While the PE class exhibits some fatty acid changes, they are small. The PS class exhibits no significant changes in fatty acid composition. The PI class which constitutes less than 2% of the total phospholipid exhibits age-related changes in each of the fatty acids which could be measured. Most notable of these is an increase in 20:4 as the disks are apically displaced. These changes indicate a remodeling of the disk membranes which may be related to the phototransduction process or to preparation for eventual disk phagocytosis. (C) 1998 Elsevier Science B.V.