Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses
Systematic Analysis of MYB Family Genes in Potato and Their Multiple Roles in Development and Stress Responses
复制标题
马铃薯 MYB 家族基因的系统分析及其在发育和应激反应中的多重作用
DOI：
10.3390/biom9080317
复制
发表时间：
2019-08-01
期刊：
影响因子：
5.5
通讯作者：
Guo, Yongfeng
中科院分区：
文献类型：
--
作者：
Li, Xiaoxu;Guo, Cun;Guo, Yongfeng
MYB 蛋白代表了一个转录因子大家族，在植物的发育、衰老和胁迫反应中发挥着重要作用。本研究在马铃薯基因组中鉴定并分析了233个MYB转录因子编码基因，其中包括119个R1-MYB、112个R2R3-MYB和2个R1R2R3-MYB成员。 R2R3-MYB 是最丰富的 MYB 亚类，根据系统发育分析的结果，马铃薯 R2R3-MYB 成员及其拟南芥同源物被分为 35 个得到充分支持的亚类。对基因结构和蛋白质基序的分析表明，来自同一亚群的成员具有相似的外显子/内含子和基序组织，进一步支持了系统发育分析的结果。通过同线分析研究了马铃薯 MYB 家族的进化。预计有 41 对 StMYB 基因由串联或片段复制事件产生，这在 StMYB 家族的扩张中发挥了重要作用。表达谱显示 StMYB 基因在多种组织中表达，并且多个 StMYB 基因被鉴定为由不同应激条件诱导。值得注意的是，StMYB030被发现与AtMYB44同源，并且通过盐和干旱胁迫处理显着上调。此外，拟南芥中StMYB030的过度表达增强了转基因植物的盐胁迫耐受性。这项研究的结果为进一步的功能分析和通过对这些 StMYB 基因进行遗传操作来改良作物提供了信息。
The MYB proteins represent a large family of transcription factors and play important roles in development, senescence, and stress responses in plants. In the current study, 233 MYB transcription factor-encoding genes were identified and analyzed in the potato genome, including 119 R1-MYB, 112 R2R3-MYB, and two R1R2R3-MYB members. R2R3-MYB is the most abundant MYB subclass and potato R2R3-MYB members together with their Arabidopsis homologs were divided into 35 well-supported subgroups as the result of phylogenetic analyses. Analyses on gene structure and protein motif revealed that members from the same subgroup shared similar exon/intron and motif organization, further supporting the results of phylogenetic analyses. Evolution of the potato MYB family was studied via syntenic analysis. Forty-one pairs of StMYB genes were predicted to have arisen from tandem or segmental duplication events, which played important roles in the expansion of the StMYB family. Expression profiling revealed that the StMYB genes were expressed in various tissues and several StMYB genes were identified to be induced by different stress conditions. Notably, StMYB030 was found to act as the homolog of AtMYB44 and was significantly up-regulated by salt and drought stress treatments. Furthermore, overexpression of StMYB030 in Arabidopsis enhanced salt stress tolerance of transgenic plants. The results from this study provided information for further functional analysis and for crop improvements through genetic manipulation of these StMYB genes.