Human decellularized adipose tissue scaffold as a model for breast cancer cell growth and drug treatments
Human decellularized adipose tissue scaffold as a model for breast cancer cell growth and drug treatments
复制标题
DOI：
10.1016/j.biomaterials.2014.03.003
复制
发表时间：
2014-06-01
期刊：
影响因子：
14
通讯作者：
An, Zhigiang
中科院分区：
文献类型：
--
作者：
Dunne, Lina W.;Huang, Zhao;An, Zhigiang
人脂肪组织细胞外基质，通过脱细胞化处理，已被证明提供一个仿生微环境的脂肪组织再生。本研究报告了使用人脂肪组织来源的细胞外基质（hDAM）支架作为三维细胞培养系统，用于乳腺癌生长和药物治疗的研究。hDAM支架具有与乳腺组织微环境相似的细胞外基质组成。乳腺癌细胞在hDAM支架中培养，并研究细胞增殖、迁移、形态和药物反应。在hDAM支架上培养的多种乳腺癌细胞系的生长特征与在二维表面上培养的细胞系的生长不同，并且更接近于异种移植物的生长。hDAM培养的乳腺癌细胞在细胞形态、迁移、粘附分子表达和对药物治疗的敏感性方面也不同于在二维表面上培养的乳腺癌细胞。我们的研究结果表明，hDAM系统在体外为乳腺癌细胞提供了一个仿生微环境，比现有的二维和Matrigel三维培养物更接近地模拟体内微环境，从而可以为癌细胞的表征和癌症治疗药物的筛选提供重要信息。(C)2014爱思唯尔有限公司版权所有。
Human adipose tissue extracellular matrix, derived through decellularization processing, has been shown to provide a biomimetic microenvironment for adipose tissue regeneration. This study reports the use of human adipose tissue-derived extracellular matrix (hDAM) scaffolds as a three-dimensional cell culturing system for the investigation of breast cancer growth and drug treatments. The hDAM scaffolds have similar extracellular matrix composition to the microenvironment of breast tissues. Breast cancer cells were cultured in hDAM scaffolds, and cell proliferation, migration, morphology, and drug responses were investigated. The growth profiles of multiple breast cancer cell lines cultured in hDAM scaffolds differed from the growth of those cultured on two-dimensional surfaces and more closely resembled the growth of xenografts. hDAM-cultured breast cancer cells also differed from those cultured on two-dimensional surfaces in terms of cell morphology, migration, expression of adhesion molecules, and sensitivity to drug treatment. Our results demonstrated that the hDAM system provides breast cancer cells with a biomimetic microenvironment in vitro that more closely mimics the in vivo microenvironment than existing two-dimensional and Matrigel three-dimensional cultures do, and thus can provide vital information for the characterization of cancer cells and screening of cancer therapeutics. (C) 2014 Elsevier Ltd. All rights reserved.