A YEAST ORIGIN OF REPLICATION IS ACTIVATED LATE IN S-PHASE
A YEAST ORIGIN OF REPLICATION IS ACTIVATED LATE IN S-PHASE
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DOI：
10.1016/0092-8674(91)90468-e
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发表时间：
1991-05-03
期刊：
影响因子：
64.5
通讯作者：
FANGMAN, WL
中科院分区：
文献类型：
--
作者：
FERGUSON, BM;BREWER, BJ;FANGMAN, WL
导致真核细胞染色体大部分区域直到S期后期才复制的机制尚不清楚。 我们发现，S.酿酒酵母在S期后期复制。 二维凝胶分析表明，该区域的ARS元件ARS 501是一个活跃的复制起点。 动力学分析表明，复制叉运动的速度在这个晚期区域内是类似的早期复制区域。 因此，该区域的延迟复制是晚期起源激活的结果。 这些结果也支持这样的想法，即散布早期和晚期复制沿着高等真核生物的染色体的模式是一个结果的时间调节的起源激活。
The mechanism that causes large regions of eukaryotic chromosomes to remain unreplicated until late in S phase is not understood. We have found that 67 kb of telomere-adjacent DNA at the right end of chromosome V in S. cerevisiae is replicated late in S phase. An ARS element in this region, ARS501, was shown by two-dimensional gel analysis to be an active origin of replication. Kinetic analyses indicate that the rate of replication fork movement within this late region is similar to that in early replicating regions. Therefore, the delayed replication of the region is a consequence of late origin activation. The results also support the idea that the pattern of interspersed early and late replication along the chromosomes of higher eukaryotes is a consequence of the temporal regulation of origin activation.