Responses of microbial carbon metabolism and function diversity induced by complex fungal enzymes in lignocellulosic waste composting
Responses of microbial carbon metabolism and function diversity induced by complex fungal enzymes in lignocellulosic waste composting
复制标题
复合真菌酶诱导木质纤维素废物堆肥中微生物碳代谢和功能多样性的响应
DOI：
10.1016/j.scitotenv.2018.06.102
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发表时间：
2018
影响因子：
9.8
通讯作者：
Zeng Guangming
中科院分区：
文献类型：
--
作者：
Zeng Zhuotong;Guo Xueying;Xu Piao;Xiao Rong;Huang Danlian;Gong Xiaomin;Cheng Min;Yi Huan;Li Tao;Zeng Guangming
堆肥是一种经济有效的固体废物处理技术，是促进污染物生物地球化学循环的重要方法。然而，该技术的应用受到木质素难生物降解和其他植物毒性物质释放的限制。与微生物和酶的结合是一种流行且有效的增强堆肥的方法。本研究参考代谢机制、真菌分子和生物地球化学循环，研究了木质素降解、碳代谢多样性的影响，以及这两种复合酶在堆肥过程中引起的相关变化。生物多样性是生态系统的重要指标，关系到一项技术的环境适用性。碳代谢多样性反映了有机物的生物地球化学循环，这也是分析堆肥效果的重要输入。碳多样性特征的变化对于全面了解这一过程的深层机制、拓展复合酶在强化堆肥领域的应用具有重要意义。 Biolog分析表明，堆肥中添加复合酶后，丙酮酸甲酯、α-环糊精、d-甘露醇、d-半乳糖醛酸、衣康酸和l-天冬酰胺的利用率得到了深度促进，而ofd、l-α-磷酸甘油、l-苏氨酸、甘氨酰-l-谷氨酸和腐胺的利用率得到了抑制。此外，数据显示，复合酶的添加提高了堆肥中碳的降解效率和代谢能力。这些发现无疑有助于酶技术的发展和复合酶在堆肥中的应用，对于消除堆肥的局限性和扩大堆肥的应用具有很大的好处。
Composting is an economic and effective technology for solid waste treatment, which is an essential method to promote the biogeochemical cycle of contaminants. However, the application of this technology was limited by the bio-degradative recalcitrance of lignin and other kind of phytotoxic substances release. The combination with microorganisms and enzymes is a popular and efficient way to enhanced composting. This study, referring to metabolic mechanisms, fungal molecular and biogeochemical cycles, was performed to investigate the effects of lignin degradation, carbon metabolic diversity, as well as the related changes induced by these two kinds of complex enzymes in composting. The biological diversity is important indicator in ecosystem, which concerns the environmental applicability of one technology. The carbon metabolism diversity reflected the biogeochemical cycles of organic matter, which was also an essential input to analyze the effects of composting. The changes on the diversity characteristics of carbon are essential to comprehensively understand the deep mechanisms of this process, and extended the application of complex enzymes in the field of enhanced composting. The analysis of Biolog revealed that the utilization of pyruvic acid methyl ester, α-Cyclodextrin,d-Mannitol,d-Galacturonic, Itaconic acid andl-asparagine were deeply promoted, and that ofd,l-α-Glycerol-phosphate,l-Threonine, Glycyl-l-Glutamic acid and putrescine were depressed by adding the complex enzyme in composting. Moreover, according to the data, the addition of complex enzymes improved the degradation efficiency and the metabolic capacity of carbon in composting. These findings undoubtedly contribute to the development of enzyme-based technologies and the applications of complex enzymes in composting, which is of great benefit to eliminate the limitation and extend the application of composting.