Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.
Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.
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DOI：
10.1002/0471142700.nc0125s47
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发表时间：
2011-12
影响因子：
--
通讯作者：
Huang Z
中科院分区：
文献类型：
--
作者：
Jiang S;Sheng J;Huang Z
In this unit, an efficient method for the synthesis of 2’-tellerium modified phosphoramidite and its incorporation into oligonucleotide are presented. We choose 5’-O-DMTr-2,2’-anhydro-uridine and -thymidine nucleosides (S.1, S.2) as starting materials due to their easy preparation. The 5’-O-DMTr-2,2’-anhydro-uridine and -thymidine can be converted to corresponding the 2’-tellerium-derivatized nucleosides by treating with the telluride nucleophiles. Subsequently, the 2’-Te-nucleosides can be transformed into 3’-phosphoramidites, which are the building blocks for DNA/RNA synthesis. The DNA synthesis, purification and applications of oligonucleotides containing 2’-Te-U or 2’-Te-T are described in this protocol.
