Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge
Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge
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DOI：
10.1016/j.watres.2004.04.047
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发表时间：
2004-08-01
期刊：
影响因子：
12.8
通讯作者：
Noguera, DR
中科院分区：
文献类型：
--
作者：
Park, HD;Noguera, DR
以amoA基因作为系统发育比较的基础，研究了溶解氧（DO）对活性污泥中氨氧化细菌（AOB）群落的影响.在控制的实验室条件下，两个恒化器接种活性污泥从相同的来源，高溶解氧（8.5mg/L）和低溶解氧（0.24和0.12 mg/L）浓度运行300天。在运行期结束时，恒化器丰富的AOB社区，属于亚硝化单胞菌europaea血统，但可区分的基础上系统发育和动力学分析。低DO恒化器在该谱系内具有两个不同组的生长，可通过amoA序列比较和末端片段特征来区分。通过评估作为氧浓度的函数的生长动力学来证明高DO和低DO富集之间的氧亲和力的差异。低溶解氧富集在溶解氧浓度低于4.7 mg/L时具有较高的生长速率，但在较高溶解氧浓度下生长速率显著降低，高溶解氧富集经历较高的生长速率。此外，在一个全面的处理厂内的两个平行的列车中的AOB种群的动态变化进行了评估，以响应显着减少的溶解氧在一个处理列车。只有在DO浓度低于1 mg/L的条件下，才有利于与N.欧洲血统。(C)2004爱思唯尔有限公司保留所有权利。
The effect of dissolved oxygen (DO) on the communities of ammonia-oxidizing bacteria (AOB) in activated sludge was evaluated in lab-scale and full-scale reactors using the amoA gene as the basis for phylogenetic comparisons. Under controlled laboratory conditions, two chemostats seeded with activated sludge from the same source were operated with high-DO (8.5mg/L) and low-DO (0.24 and 0.12 mg/L) concentrations for a period of 300 days. At the end of the operation period, the chemostats had enriched AOB communities that belonged to the Nitrosomonas europaea lineage, but were differentiable based on phylogenetic and kinetic analyses. The low-DO chemostat harbored the growth of two different groups within this lineage, differentiable by the amoA sequence comparison and by terminal fragment signatures. The difference in oxygen affinity between high-DO and low-DO enrichments was demonstrated by evaluating the growth kinetics as a function of oxygen concentration. The low-DO enrichment had a higher growth rate at DO concentrations below 4.7 mg/L, but the growth rate significantly decreased at higher DO concentrations, for which the high-DO enrichment experienced higher growth rates. In addition, the dynamic changes in AOB populations in two parallel trains within one full-scale treatment plant were evaluated in response to a significant reduction of DO in one of the treatment trains. Only the train operated with DO concentrations below 1 mg/L favored the establishment of a population of AOB related to the N. europaea lineage. (C) 2004 Elsevier Ltd. All rights reserved.