Neurokinin B signaling in hermaphroditic species, a study of the orange-spotted grouper (Epinephelus coioides)
Neurokinin B signaling in hermaphroditic species, a study of the orange-spotted grouper (Epinephelus coioides)
复制标题
雌雄同体物种中的神经激肽 B 信号转导，对橙斑石斑鱼 (Epinephelus coioides) 的研究
DOI：
10.1016/j.ygcen.2018.01.009
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发表时间：
2018-05-01
影响因子：
2.7
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Chen, Huapu;Xiao, Ling;Lin, Haoran
神经激肽 B (NKB) 通过调节促性腺激素释放激素 (GnRH) 和促性腺激素的释放，在哺乳动物生殖轴中发挥重要作用。在本研究中，tac3 cDNA 是从雌雄同体物种——橙斑石斑鱼中克隆出来的。序列分析表明石斑鱼Tac3前体编码两种速激肽，NKB和NKB相关肽（NKBRP）。不同组织中的表达分析表明，tac3 mRNA在橙斑石斑鱼的大脑中高表达。原位杂交进一步揭示，它位于一些与生殖调节相关的下丘脑核团中。在卵巢发育过程中，在卵黄发生阶段观察到下丘脑中 tac3 表达增加。腹腔注射NKB可增加gnrhl和lh beta mRNA水平，并提高血清雌激素水平，但对培养的垂体细胞中110的表达没有显着影响，表明NKB并不直接对垂体发挥作用。但发现NKBRP在体内外对两个gnrhs和两个gths的表达没有影响。进一步研究了性类固醇对 tac3 表达的影响。在17-甲基睾酮诱导橙斑石斑鱼性别变化的过程中，下丘脑tac3的表达没有表现出明显的变化。有趣的是，卵巢切除术极大地刺激了 tac3 的表达，而 17β-雌二醇治疗则逆转了这种效应。总的来说，我们的数据高度表明 NKB 信号可以激活橙斑石斑鱼的生殖轴。我们的研究首次描述了雌雄同体物种中的 NKB 信号传导。 (C) 2018 Elsevier Inc. 保留所有权利。
Neurokinin B (NKB) plays important roles in the mammalian reproductive axis by modulating the release of gonadotropin-releasing hormone (GnRH) and gonadotropins. In the present study, the tac3 cDNA was cloned from a hermaphroditic species, the orange-spotted grouper. Sequence analysis showed that the grouper Tac3 precursor encoded two tachykinin peptides, NKB and NKB-related peptide (NKBRP). Expression analysis in different tissues revealed that tac3 mRNA was highly expressed in the brain of the orange-spotted grouper. In situ hybridization further revealed that it was localized in some hypothalamic nuclei associated with reproductive regulation. During ovarian development, an increase of tac3 expression in the hypothalamus was observed at vitellogenesis stage. Intraperitoneal administration of NKB could increase the gnrhl and lh beta mRNA levels, and enhance the serum estrogen levels, but did not significantly influence 110 expression in cultured pituitary cells, indicating that NKB does not directly exert its actions on the pituitary gland. However, it was found that NKBRP had no effect on the expression of two gnrhs and two gths in vivo and in vitro. Effects of sex steroids on tac3 expression were further investigated. During the 17-methyltestosterone-induced sex change in the orange-spotted grouper, hypothalamic tac3 expression showed no significant change. Interestingly, ovariectomy greatly stimulated tac3 expression, while the 17 beta-estradiol treatment reversed this effect. In general, our data highly indicated that NKB signaling could activate the reproductive axis in the orange-spotted grouper. Our study is the first description of the NKB signaling in the hermaphroditic species. (C) 2018 Elsevier Inc. All rights reserved.