Carbon supply and the regulation of enzyme activity in constructed wetlands
Carbon supply and the regulation of enzyme activity in constructed wetlands
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DOI：
10.1016/s0038-0717(00)00169-3
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发表时间：
2000-11
影响因子：
9.7
通讯作者：
V. Shackle;C. Freeman;B. Reynolds
中科院分区：
文献类型：
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作者：
V. Shackle;C. Freeman;B. Reynolds
人工湿地（即用于废水处理的人工湿地）中细胞外酶活性的调节尚未阐明；这项工作的目的是调查一种可能的调节剂（碳供应）对参与有机物分解的三种酶的活性速率的贡献。来自人工湿地的土壤样本提供了不同浓度（10 和 100mgl−1）的碳（纤维素或葡萄糖）以及无碳水（对照）或污水。使用仅在细胞外裂解的甲基伞形酮底物对土壤中 β-d-葡萄糖苷酶、磷酸酶和芳基硫酸酯酶的活性进行了 54 天的监测（Jones 1990）。与不接受碳的对照相比，所有处理下的磷酸酶活性在实验过程中没有显示出显着差异。在实验过程中，添加葡萄糖（10 和 100mgl−1）、纤维素（10 和 100mgl−1）以及污水废水，硫酸酶活性显着降低，降至对照活性的 53% 到 79%。随着纤维素的添加（10 和 100mgl−1），β-葡萄糖苷酶活性在 54 天内逐渐增加 127-161%，而在添加污水后，β-葡萄糖苷酶活性下降至对照活性的 84%。这些发现表明，通过控制人工湿地中碳供应的数量和质量，有可能改变细胞外酶的活性，从而最大限度地提高水处理效率。
Regulation of extracellular enzyme activity in constructed wetlands (i.e. artificial wetlands for wastewater treatment) has not been elucidated; the objective of this work was to investigate the contribution of one possible regulator (carbon supply) to rates of activity of three enzymes involved in organic matter decomposition. Soil samples from a constructed wetland were supplied with carbon (cellulose or glucose) at different concentrations (10 and 100mgl−1) and with carbon-free water (control) or sewage effluent. The activities of β-d-glucosidase, phosphatase and arylsulphatase in the soil were monitored over a 54-day period using methylumbelliferyl substrates, which are cleaved only extracellularly (Jones 1990). When compared to controls receiving no carbon, phosphatase activity under all treatments showed no significant differences over the course of the experiment. Sulphatase activities over the course of the experiment were significantly reduced by glucose (10 and 100mgl−1), cellulose (10 and 100mgl−1) and sewage effluent addition, to between 53 and 79% of the activity in controls. β-glucosidase activity gradually increased by 127–161% over the 54 d in response to cellulose addition (10 and 100mgl−1), and decreased after sewage effluent addition to 84% of the activity in controls. These findings suggest that by manipulating the quantity and quality of carbon supply in constructed wetlands, it may be possible to modify extracellular enzyme activities in order to maximize the efficiency of water treatment.