A prolactin-inducible T cell gene product is structurally similar to the Aspergillus nidulans nuclear movement protein NUDC
A prolactin-inducible T cell gene product is structurally similar to the Aspergillus nidulans nuclear movement protein NUDC
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DOI：
10.1210/me.11.2.229
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发表时间：
1997-02-01
影响因子：
--
通讯作者：
YuLee, LY
中科院分区：
文献类型：
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作者：
Morris, SM;Anaya, P;YuLee, LY
克隆15（clone 15，c15）最初被鉴定为活化T细胞中的催乳素诱导基因，序列分析表明c15的后94个氨基酸与丝状真菌构巢曲霉核运动蛋白NUDC的同源性为68%，相似性为78%。哺乳动物（大鼠）c15蛋白的鉴定表明，羧基末端NUDC样区域在进化过程中具有重要的结构和/或功能。为了确定c15是否在功能上类似于NUDC，使用诱导型/抑制型pAL 5载体系统进行互补研究。互补实验的结果表明，全长哺乳动物c15蛋白不仅能够挽救nudC 3突变体的核运动缺陷，而且还能够将下游内源NUDF蛋白的表达恢复到接近野生型水平。这些结果表明，大鼠c15和真菌NUDC不仅具有相似的结构，而且还具有相似的功能。综上所述，c15和NUDC之间的结构和功能保守性与c15是nudC的大鼠同源物的概念一致，因此被命名为RnudC。
Clone 15 (c15) was originally identified as a PRL-inducible gene in activated T cells, Sequence analysis of c15 revealed that the last 94 amino acids of c15 are 68% identical and 78% similar to the filamentous fungus Aspergillus nidulans nuclear movement protein NUDC. The identification of the mammalian (rat) c15 protein suggests that the carboxy-terminal NUDC-like region has been conserved over evolution for an important structure and/or function. To determine whether c15 is functionally analogous to NUDC, complementation studies were performed using the inducible/repressible pAL5 vector system. The results of the complementation experiments show that the full-length mammalian c15 protein is not only capable of rescuing the nuclear movement defect of the nudC3 mutants, but is also able to restore the expression of the downstream endogenous NUDF protein to near wild type levels. These results indicate that rat c15 and fungal NUDC not only share similar structures, but also serve similar functions, Taken together, the structural and functional conservation between c15 and NUDC is consistent with the notion that c15 is the rat homolog of nudC and has therefore been given the name RnudC.