FURTHER CHARACTERIZATION OF MESSENGER-RNAS OF MOUSE HEPATITIS-VIRUS - PRESENCE OF COMMON 5'-END NUCLEOTIDES
FURTHER CHARACTERIZATION OF MESSENGER-RNAS OF MOUSE HEPATITIS-VIRUS - PRESENCE OF COMMON 5'-END NUCLEOTIDES
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DOI：
10.1128/jvi.41.2.557-565.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
STOHLMAN, SA
中科院分区：
文献类型：
--
作者：
LAI, MMC;PATTON, CD;STOHLMAN, SA
小鼠肝炎病毒A59株在感染的小鼠L细胞中编码7种RNA。发现这些病毒特异性RNA与多核糖体相关，因此可能代表mRNA。它们都有共同的3“-末端序列。它们的结构相对于它们的5“-末端序列被进一步研究。所有这些mRNa在它们的5“末端含有帽结构。发现代表紧邻5“末端的序列的含帽寡核苷酸对于7种病毒特异性mRNA中的大多数（如果不是全部）是相同的。这些序列也与病毒体RNA基因组的5“-末端序列相同。5“-端序列初步确定为5”-cap-N-UAAG。小鼠肝炎病毒株A59中所有病毒特异性RNA中存在共同核苷酸，提示了这些RNA合成的几种可能机制。这些发现的意义进行了讨论。
The mouse hepatitis virus strain A59 codes for 7 RNA species in infected mouse L cells. These virus-specific RNA were found to be polysome associated and therefore likely to represent mRNA. All have common 3''-end sequences. Their structure was further studied with respect to their 5''-end sequences. All of these mRNa contained cap structures at their 5'' ends. The cap-containing oligonucleotides which represent the sequences immediately adjacent to the 5'' ends were found to be the same for most, if not all, of the 7 virus-specific mRNA. These sequences are also identical to the 5''-end sequences of the virion RNA genome. The 5''-end sequences were tentatively determined to be 5''-cap-N-UAAG. The presence of the common nucleotides in all of the virus-specific RNA in mouse hepatitis virus strain A59 suggests several possible mechanisms of synthesis for these RNA. The significance of these findings is discussed.